AP26045PU-S CHK2 (514-518) antibody

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50 µg / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human CHK2

Product Description for CHK2

Rabbit anti Human CHK2.
Properties: (514-518)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for CHK2

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
O96017
Immunogen:
Peptide sequence around aa 514~518 derived from Chk2
AA Sequence:
Q-P-S-T-S
GeneID:
11200
Property 514-518
Add. information Molecular weight: 62 kDa
Application Western blot: 1:500 - 1:1000.
Background The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
Concentration 1.0 mg/ml
General Readings
  1. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
  2. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
  3. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.
Storage Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity-chromatography using epitope-specific peptide
Buffer System:
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
State:
Liquid Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human
Specificity
Specificity:
This antibody detects endogenous levels of total Chk2 protein.
Gene ID 11200

Accessory Products

Proteins and/or Positive Controls

Proteins for CHK2 (6 products)

Catalog No. Species Pres. Purity   Source  

CHK2 (transcript variant 1)

CHK2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human
  Abnova Taiwan Corp.

Positive controls for CHK2 (4 products)

Catalog No. Species Pres. Purity   Source  

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

Chk2 Lysate

Western Blot: Chk2 Lysate [NBL1-09147] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for CHEK2.
  Novus Biologicals Inc.
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