TA333183 CHK2 antibody

Phospho-CHEK2-S516 Rabbit Polyclonal Antibody

See related secondary antibodies

Search for all "CHK2"

0.1 mg / €335.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human CHK2

Product Description for CHK2

Rabbit anti Human CHK2.
Presentation: Purified

Properties for CHK2

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Presentation Purified
Reactivity Hu
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O96017
Immunogen:
A phospho specific peptide corresponding to residues surrounding S516 of human CHEK2.
GeneID:
11200
Application WB 1:500 - 1:2000
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene.
Format
Purification:
Affinity purification
Buffer System:
Store at -20°C (regular) and -80°C (long term). Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
State:
Purified

Accessory Products

Proteins and/or Positive Controls

Proteins for CHK2 (6 products)

Catalog No. Species Pres. Purity   Source  

CHK2 (transcript variant 1)

CHK2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €438.00
  OriGene Technologies, Inc.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human 12.5% SDS-PAGE Stained with Coomassie Blue
  Abnova Taiwan Corp.

CHK2

CHK2 Human
  Abnova Taiwan Corp.

Positive controls for CHK2 (4 products)

Catalog No. Species Pres. Purity   Source  

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)

CHEK2 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CHEK2 overexpression lysate

CHEK2 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

Chk2 Lysate

Western Blot: Chk2 Lysate [NBL1-09147] - Western Blot experiments.  Left-Control; Right -Over-expression Lysate for CHEK2.
  Novus Biologicals Inc.
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