NB100-75152 Cholera enterotoxin subunit B antibody

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Chicken anti Bacteria Cholera enterotoxin subunit B


Product Description for Cholera enterotoxin subunit B

Chicken anti Bacteria Cholera enterotoxin subunit B.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Cholera enterotoxin subunit B

Product Category Primary Antibodies
Quantity 0.05 mg
Presentation Aff - Purified
Reactivity Bac
Applications WB
Clonality Polyclonal
Host Chicken
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

The immunogen for this antibody was a sequence from 1a.a. to 124a.a. from Cholera enterotoxin subunit B(a 124a.a. long protein)
Application Western Blot
Background The B subunit of cholera toxin (CtxB) binds to a GM1-ganglioside receptor, a ubiquitous glycolipid cell surface receptor. This binding is widely accepted to initiate toxin action by triggering uptake and delivery of the toxin A subunit into cells. The beta chain has no toxic activity by itself. The holotoxin consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.
The A subunit (and Cholera toxin) activates the adenylate cyclase enzyme in cells of the intestinal mucosa leading to increased levels of intracellular cAMP.

The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
General Readings
  1. Lebens,M. and Holmgren,J., et al.. Structure and arrangement of the cholera toxin genes in Vibrio cholerae O139
  2. Lebens,M., et al.. Direct Submission
  3. , Lockman H., Kaper J.B.. Nucleotide sequence analysis of the A2 and B subunits of Vibrio cholerae enterotoxin.
  4. , Mekalanos J.J., Swartz D.J., Pearson G.D.N., Harford N., Groyne F., de Wilde M.. Cholera toxin genes: nucleotide sequence, deletion analysis and vaccine development.
  5. , Dams E., de Wolf M., Dierick W.. Submitted (MAY-1991) to the EMBL/GenBank/DDBJ databases.
  6. , Lebens M., Holmgren J.. Structure and arrangement of the cholera toxin genes in Vibrio cholerae O139.
  7. , Yamamoto K., Do V.G.R.F., Xu M., Iida T., Miwatani T., Albert M.J., Honda T.. Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
  8. , Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., et al.. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae.
  9. , Kurosky A., Markel D.E., Peterson J.W.. Covalent structure of the beta chain of cholera enterotoxin.
  10. , Lai C.-Y.. Determination of the primary structure of cholera toxin B subunit.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
Phosphate-Buffered Saline.
Immunogen affinity purified
Aff - Purified
This antibody is reactive against Bacteria.

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