discontinued

NB100-66405 CHOLERA EXOTOXIN antibody

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1 ml / €350.00

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Goat anti Bacteria CHOLERA EXOTOXIN

NB100-66405

Product Description for CHOLERA EXOTOXIN

Goat anti Bacteria CHOLERA EXOTOXIN.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for CHOLERA EXOTOXIN

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Bac
Applications E, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Purified, intact cholera toxin from Vibrio cholera, type Inaba 569B.
Application Western Blot, ELISA
Background The holotoxin (choleragen) consists of a pentameric ring of B subunits whose central pore is occupied by the A subunit. The A subunit contains two chains, A1 and A2, linked by a disulfide bridge.
The B subunit pentameric ring directs the A subunit to its target by binding to the GM1 gangliosides present on the surface of the intestinal epithelial cells. It can bind five GM1 gangliosides. It has no toxic activity by itself.
After binding to gangliosides GM1 in lipid rafts, through the subunit B pentamer, the holotoxin and the gangliosides are internalized. The holotoxin remains bound to GM1 until arrival in the ER. The A subunit has previously been cleaved in the intestinal lumen but the A1 and A2 chains have remained associated. In the ER, the A subunit disulfide bridge is reduced, the A1 chain is unfolded by the PDI and disassembled from the rest of the toxin. Then, the membrane-associated ER oxidase ERO1 oxidizes PDI, which releases the unfolded A1 chain. The next step is the retrotranslocation of A1 into the cytosol. This might be mediated by the protein-conducting pore SEC61. Upon arrival in the cytosol, A1 refolds and avoids proteasome degradation. In one way or another, A1 finally reaches its target and induces toxicity.
Concentration 5.0mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
protein G purified
Buffer System:
Phosphate buffered saline, pH7.4
Purity:
protein G purified
State:
Purified
Specificity
Species:
Bacteria. Not yet tested in other species.

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