discontinued

R1075HRP Cholesterol Oxidase antibody

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20 mg / €3,300.00

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Goat anti Bacteria Cholesterol Oxidase

R1075HRP

Product Description for Cholesterol Oxidase

Goat anti Bacteria Cholesterol Oxidase.
Presentation: HRP
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Cholesterol Oxidase

Product Category Primary Antibodies
Quantity 20 mg
Synonyms CHOD, Cholesterol oxidase, EC 1.1.3.6
Presentation HRP
Reactivity Bac
Applications E, WB
Clonality Polyclonal
Host Goat
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Cholesterol Oxidase [Microorganism].
Application Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions: This product has been assayed against 1.0 ug of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Background Cholesterol Oxidases exist as both type I and type II oxidases and are implicated in bacterial pathogenesis. In addition, they are important as clinical reagents, potential larvicides, and tools in cell biology.
Caution Do Not Use Sodium Azide as Preservative.
Concentration 10.0 mg/ml (by UV absorbance at 280 nm)
General Readings Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981. (Conjugation)
Storage Store vial at 2-8°C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
This product is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.
Shelf life: One year from despatch.
Format
Label:
Horseradish Peroxidase
HRP
Purification:
Multi-step process.
Buffer System:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Gentamicin Sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
State:
Lyophilized purified Ig fraction.
Reconstitution:
Restore with 2.0 ml of deionized water (or equivalent).
Specificity
Specificity:
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].
Cross reactivity against Cholesterol Oxidase from other sources is unknown.

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