AM32584SU-N Chondroitin-6-Sulphate antibody

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Mouse anti Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep Chondroitin-6-Sulphate MK-302


Product Description for Chondroitin-6-Sulphate

Mouse anti Bovine, Canine, Human, Mouse, Rabbit, Rat, Sheep Chondroitin-6-Sulphate MK-302.
Presentation: Ascites
Product is tested for Radioimmunoassay, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Chondroitin-6-Sulphate

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Ascites
Reactivity Bov, Can, Hu, Ms, Rb, Rt, Sh
Applications E, R, WB
Clonality Monoclonal
Clone MK-302
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Chondroitinase ABC-digested adult Human Aggrecan.
Application ELISA: 1/100-1/5,000. 
RIA: 1/100-1/200.
Chondroitinase ABC digestion prior to antibody reaction is required for antibody reactivity. {0.5U/mL in 100mM Tris-HCL, 30 minutes room temperature} A dramatic proteolytic digestion of the core protein (e.g. with papain or pronase) significantly reduces the antibody reactivity.
General Readings
  1. Glant TT, Mikecz K, Poole AR. Monoclonal antibodies to different protein-related epitopes of human articular cartilage proteoglycans. Biochem J. 1986 Feb 15;234(1):31-41. PubMed PMID: 2423072. (Free PMC Article available)
  2. Arthritis Rheum. (1987). 30:306-318.
  3. Poole CA, Glant TT, Schofield JR. Chondrons from articular cartilage. (IV). Immunolocalization of proteoglycan epitopes in isolated canine tibial chondrons. J Histochem Cytochem. 1991 Sep;39(9):1175-87. PubMed PMID: 1717545.
  4. Glant TT, Buzás EI, Finnegan A, Negroiu G, Cs-Szabó G, Mikecz K. Critical roles of glycosaminoglycan side chains of cartilage proteoglycan (aggrecan) in antigen recognition and presentation. J Immunol. 1998 Apr 15;160(8):3812-9. PubMed PMID: 9558085.
  5. Nicodemus GD, Skaalure SC, Bryant SJ. Gel structure has an impact on pericellular and extracellular matrix deposition, which subsequently alters metabolic activities in chondrocyte-laden PEG hydrogels. Acta Biomater. 2011 Feb;7(2):492-504. doi: 10.1016/j.actbio.2010.08.021. Epub 2010 Sep 8. PubMed PMID: 20804868. (Free PMC Article available, 7 images available)
  6.  Hang Wang,Yasuhiro Katagiri,Thomas E McCann,Edward Unsworth,Paul Goldsmith,Zu-Xi Yu,Fei Tan,Lizzie Santiago,Edward M Mills,Yu Wang,Aviva J Symes,Herbert M Geller (2008) Journal of cell science.121. 
  7.  Yvette M Coulson-Thomas,Vivien J Coulson-Thomas,Thais R Filippo,Renato A Mortara,Rafael B da Silveira,Helena B Nader,Marimélia A Porcionatto (2008) Journal of neuroscience methods.171.

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Liquid Ascites
This antibody is not species specific, thus, it recognizes Chondroitin-6-Sulfate stubs of all proteoglycans of all species. 
Reacts with a wide range of cartilage proteoglycans after either chondroitinase (ABC or AC) or testicular hyaluronidase digestion. Native proteoglycans do not react with this antibody.
Antibody binding to epitope is successfully inhibited by disaccharides of Chondroitin-6-Sulfate.
Recognizes the Chondroitin-6-Sulfate stubs (preferentially in clusters) of high density cartilage proteoglycans of different species.
No cross-reactivity with dermatan sulfate, keratan sulfate or Chondroitin-4-sulfate.
Bovine, Canine, Human, Mouse, Rat, Rabbit, Sheep.

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