NB100-78039 CLA antibody

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Rat anti Human, Mouse CLA HECA-452

Product Description for CLA

Rat anti Human, Mouse CLA HECA-452.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections.

Properties for CLA

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cutaneous lymphocyte-associated antigen
Presentation Aff - Purified
Reactivity Hu, Ms
Applications C, F, P
Clonality Monoclonal
Clone HECA-452
Host Rat
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Application flow cytometry / FACS analysis, immunohistochemistry, Applications include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes., flow cytometry / FACS analysis immunohistochemistry
Background Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA, and is suitable for staining formalin-fixed paraffin embedded tissue sections.
General Readings
  1. Picker, L. J., et al., 1990. Am. J. Pathol. 136: 10532. Berg, E. L., et al., 1991. J. Exp. Med. 174: 14613. Fuhlbrigge, R. C., et al., 1997. Nature 389: 978
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
0.09% Sodium Azide
Immunogen affinity purified
Aff - Purified
CLA (HECA-452)
Human, Mouse

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