NB100-78041 CLA antibody

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Rat anti Human, Mouse CLA HECA-452

Product Description for CLA

Rat anti Human, Mouse CLA HECA-452.
Presentation: FITC
Product is tested for Frozen Sections, Flow Cytometry, Paraffin Sections, Neutralisation.

Properties for CLA

Product Category Primary Antibodies
Quantity 100 Tests
Synonyms Cutaneous lymphocyte-associated antigen
Presentation FITC
Reactivity Hu, Ms
Applications C, F, NEUT, P
Clonality Monoclonal
Clone HECA-452
Host Rat
Isotype IgM
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Add. information Research Areas: Lipid and Metabolism
Application flow cytometry / FACS analysis, Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microliter per million cells or 20 microliter per 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application., flow cytometry / FACS analysis
Background Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb HECA-452. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. The HECA-452 antibody is cross-reactive with mouse CLA, and is suitable for staining formalin-fixed paraffin embedded tissue sections.
General Readings
  1. Picker, L. J., et al., 1990. Am. J. Pathol. 136: 10532. Berg, E. L., et al., 1991. J. Exp. Med. 174: 14613. Fuhlbrigge, R. C., et al., 1997. Nature 389: 978
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
Sodium Azide
Immunogen affinity purified
CLA (HECA-452)
Human, Mouse

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