AM00525PU-N Clathrin Heavy Chain antibody

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Mouse anti Human Clathrin Heavy Chain TD.1


Product Description for Clathrin Heavy Chain

Mouse anti Human Clathrin Heavy Chain TD.1.
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Clathrin Heavy Chain

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications WB
Clonality Monoclonal
Clone TD.1
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

The globular N-terminal domain of clathrin heavy chain
Mol. Wt. of Antigen: 192 kDa (Heavy chain)
Isotype control AM03095PU-N, SM10P (for use in human samples)
Add. information Cellular Localization: Cell membrane and cytoplasm
Application Western Blotting (2): 1-2 µg/ml for 2hrs at RT.
Positive Control: Placenta.
Not suitable for Immunhistology and Immunoprecipitation.
Background Clathrin consists of three heavy chain (192 kDa) and three light chains (LCa or LCb, 25-29 kDa) and self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.
Concentration 0.2 mg/ml
General Readings
  1. AP Jackson et al. Nature 326:154-159, 1987.
  2. IS Nathke et al. Cell 68:899-910, 1992.
Storage Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide
Liquid ascites fluid
AM00525PU-N reacts with clathrin heavy chain. It does not recognize native clathrin but is useful in immunoblotting.

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