TA306786 Claudin-1 / CLDN1 antibody

Rabbit Polyclonal CLDN1 Antibody

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0.1 mg / €371.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human, Mouse, Rat Claudin-1 / CLDN1

TA306786

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Product Description for Claudin-1 / CLDN1

Rabbit anti Human, Mouse, Rat Claudin-1 / CLDN1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Claudin-1 / CLDN1

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CLD1, PRO944, SEMP1, Senescence-associated epithelial membrane protein, UNQ481
Presentation Aff - Purified
Reactivity Hu, Ms, Rt
Applications E, ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O95832
Immunogen:
20 amino acid peptide near the carboxyl terminus of Human CLDN1 (AP30236CP-N)
GeneID:
9076
Add. information Blocking peptide available: AP30236CP-N
Application ELISA.
Western Blot: 1-2 μg/ml. HepG2 cell lysate can be used as positive control. 
Immunocytochemistry: Start at  5 µg/ml. 
Immunofluorescence:
Start at  20 µg/ml.
Background Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are specialized regions of cell to cell contact consisting of networking strands that act as a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. CLDN1 and CLDN2 were found to be overexpressed in colonal cancer tissues and may be useful as tumor markers and targets for the treatment of colorectal cancer. Characterization of Claudins expression in human tumors can be an additional diagnostic tool. Recent studies show that CLDN1 has gastric tumor suppressive activity and is a direct transcriptional target of RUNX3. Along with SCARB1, LDL-R, and the tetraspanin superfamily member CD81, CLDN1 has been reported to be an entry factor for the Hepatitis C virus.
General Readings
  1. Tepass U. Claudin complexities at the apical junctional complex. Nat Cell Biol. 2003 Jul;5(7):595-7. PubMed PMID: 12833061.
  2. Kinugasa T, Huo Q, Higashi D, Shibaguchi H, Kuroki M, Tanaka T, et al. Selective up-regulation of claudin-1 and claudin-2 in colorectal cancer. Anticancer Res. 2007 Nov-Dec;27(6A):3729-34. PubMed PMID: 17970035.
  3. Paschoud S, Bongiovanni M, Pache JC, Citi S. Claudin-1 and claudin-5 expression patterns differentiate lung squamous cell carcinomas from adenocarcinomas. Mod Pathol. 2007 Sep;20(9):947-54. Epub 2007 Jun 22. PubMed PMID: 17585317.
  4. Morohashi S, Kusumi T, Sato F, Odagiri H, Chiba H, Yoshihara S, et al. Decreased expression of claudin-1 correlates with recurrence status in breast cancer. Int J Mol Med. 2007 Aug;20(2):139-43. PubMed PMID: 17611630.
  5. Chang TL, Ito K, Ko TK, Liu Q, Salto-Tellez M, Yeoh KG, et al. Claudin-1 has tumor suppressive activity and is a direct target of RUNX3 in gastric epithelial cells. Gastroenterology. 2010 Jan;138(1):255-65.e1-3. doi: 10.1053/j.gastro.2009.08.044. Epub 2009 Aug 23. PubMed PMID: 19706291.
  6. Helle F, Dubuisson J. Hepatitis C virus entry into host cells. Cell Mol Life Sci. 2008 Jan;65(1):100-12. PubMed PMID: 17914604.
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Peptide Affinity Chromatography
Buffer System:
PBS
Preservatives:
0.02% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody recognizes CLDN1.
Species:
Human, Mouse, Rat.

Accessory Products

Proteins and/or Positive Controls

Proteins for Claudin-1 / CLDN1 (3 products)

Catalog No. Species Pres. Purity   Source  

Claudin-1 / CLDN1

Claudin-1 / CLDN1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Claudin-1 / CLDN1

Claudin-1 / CLDN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Claudin-1 / CLDN1

Claudin-1 / CLDN1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Claudin-1 / CLDN1 (3 products)

Catalog No. Species Pres. Purity   Source  

Claudin 1 Lysate

Western Blot: Claudin 1 Lysate [NBL1-09235] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CLDN1
  Novus Biologicals Inc.

CLDN1 293T Cell Transient Overexpression Lysate(Denatured)

CLDN1 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CLDN1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured)

CLDN1 HEK293 Cell Transient Overexpression Lysate (Non-Denatured) Transient overexpression cell lysate was tested with Anti-CLDN1 antibody (H00009076-M01) by Western Blots.
  Abnova Taiwan Corp.
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