DM1233 Clostridium botulinum Toxin B antibody

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0.1 mg / €330.00
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Mouse anti Clostridium botulinum Toxin B GR3G7


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Product Description for Clostridium botulinum Toxin B

Mouse anti Clostridium botulinum Toxin B GR3G7.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Flow Cytometry.

Properties for Clostridium botulinum Toxin B

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms BoNT/B, Bontoxilysin B, Bot B, Botulinum Neurotoxin type B
Presentation Purified
Reactivity Clostridium Botulinum
Applications E, F
Clonality Monoclonal
Clone GR3G7
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Genetic immunisation with cDNA encoding BoNT/B.
Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
Add. information SDS-PAGE analysis of GR-3G7:
The antibody was purified by protein G affinity chromatography from cell culture supernatants and verified by SDS-Page (Figure.3).
Application Flow cytometry: 1.2 µg/106 cells.
Cell based ELISA with intakt, transiently transfected cells: 1/200-1/400.
Background Botulinum neurotoxin type B (BoNT/B) is produced by Clostridium botulinum, a genetically diverse class of anaerobic, spore-forming, gram-positive bacilli. Seven different botulinum toxin groups have been identified serologically and are called botulinum toxin type A,B,C,D,E,F, and G. BoNT/B is a two-chain polypeptide with a 100-kDa heavy chain, which is responsible for neurospecific binding joined by a disulphide bond to a 50-kDa light chain, a zinc-endopeptidase which blocks neurotransmitter release. BoNT/B is one of the most poisonous naturally occurring substances. It inhibits acetylcholine release from neuromuscular junctions while it is used as an important therapeutic mainstay in the treatment of spasticity disorders and as a cosmetic treatment.
Concentration 2.0 mg/ml
General Readings
  1. DasGupta BR. Structure and biological activity of botulinum neurotoxin. J Physiol (Paris). 1990;84(3):220-8. PubMed PMID: 2074545.
  2. Schiavo G, Benfenati F, Poulain B, Rossetto O, Polverino de Laureto P, DasGupta BR, et al. Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin. Nature. 1992 Oct 29;359(6398):832-5. PubMed PMID: 1331807.
  3. Tonello F, Morante S, Rossetto O, Schiavo G, Montecucco C. Tetanus and botulism neurotoxins: a novel group of zinc-endopeptidases. Adv Exp Med Biol. 1996;389:251-60. PubMed PMID: 8861019.
  4. Papapetropoulos S and Singer C (2007). Botulinum toxin in movement disorders. Semin Neurol 27(2):183-94.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Affinity Chromatography on Protein G
Buffer System:
Phosphate buffered saline, pH 7.2
Liquid purified Ig fraction
Recognizes Botulinum Neurotoxin type B (BoNT/B).

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