AM26152PU-N CNF1 (C-term) antibody

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0.1 mg / €430.00
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Quick Overview

Mouse anti Escherichia coli CNF1 NG8

AM26152PU-N

Product Description for CNF1

Mouse anti Escherichia coli CNF1 NG8.
Properties: (C-term)
Presentation: Purified
Product is tested for Dot blot, Enzyme Immunoassay, Western blot / Immunoblot, Functional assay.

Properties for CNF1

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity E. coli
Applications Dot, E, FN, WB
Clonality Monoclonal
Clone NG8
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q46962
Property C-term
Isotype control AM03096PU-N
Application Immuno assays.
Western blot: 1:10 as starting dilution.
Native dot blot analyses.
Inhibition of biological activity (in vitro dilutions have to be made according to the amounts of CNF1 to be inactivated).
Background CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.
Concentration 0.1 mg/ml
General Readings
  1. Meysick KC, Mills M, O'Brien AD. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect Immun. 2001 Apr;69(4):2066-74. PubMed PMID: 11254559. (Free PMC Article available, 4 images available)
  2. McNichol BA, Rasmussen SB, Carvalho HM, Meysick KC, O'Brien AD. Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein. Infect Immun. 2007 Nov;75(11):5095-104. Epub 2007 Aug 20. PubMed PMID: 17709415. (Free PMC Article available, 6 images available)
Storage
Product should be stored at 2-8 °C.
Shelf life: one year from despatch.
Format
Purification:
Protein G purified
Buffer System:
PBS
Stabilizers:
0.1% bovine serum albumin
State:
Liquid 0.2 µm filtered Ig fraction
Purified
Specificity
Specificity:
This antibody is specific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. It recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.

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