AM32102PU-N CNF1 (704-730) antibody

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0.1 mg / €680.00
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Quick Overview

Mouse anti Escherichia coli CNF1 NG8


Product Description for CNF1

Mouse anti Escherichia coli CNF1 NG8.
Properties: (704-730)
Presentation: Liquid
Product is tested for Enzyme Immunoassay, Dot blot, Functional assay, Western blot / Immunoblot.

Properties for CNF1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cytotoxic necrotizing factor 1
Presentation Liquid
Reactivity E. coli
Applications Dot, E, FN, WB
Clonality Monoclonal
Clone NG8
Host Mouse
Isotype IgG2a
Molecular weight 115 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Synthetic peptide, corresponding to amino acids 704/730 of Escherichia coli CNF1
Property 704-730
Application Western Blot: Use 1/10 as starting working dilution.
Dot Blot
(Native conditions).
Inhibition of biological activity: In vitro dilutions have to be made according to the amounts of CNF1 to be inactivated.
Background CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.
Concentration 0.1 mg/ml
General Readings
  1. Meysick KC, Mills M, O'Brien AD. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect Immun. 2001 Apr;69(4):2066-74. PubMed PMID: 11254559. (Free PMC Article available, 4 images available)
Storage Store the antibody undiluted at 2-8°C. 
Shelf life: one year from despatch.
0.1% BSA
Liquid 0.2 µm filtered
The Monoclonal antibody clone NG8 recognizes an epitope between amino acids 704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
Gene ID 3990770

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