AM32101PU-N CNF1/CNF2 (169-191) antibody

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0.1 mg / €430.00
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Quick Overview

Mouse anti Escherichia coli CNF1/CNF2 JC4

AM32101PU-N

Product Description for CNF1/CNF2

Mouse anti Escherichia coli CNF1/CNF2 JC4.
Properties: (169-191)
Presentation: Purified
Product is tested for Functional assay, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for CNF1/CNF2

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cytotoxic necrotizing factor 1, Cytotoxic necrotizing factor 2
Presentation Purified
Reactivity E. coli
Applications E, FN, WB
Clonality Monoclonal
Clone JC4
Host Mouse
Isotype IgG2a
Molecular weight 115 kDa
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q1R2U0
GeneID:
3990770
Property 169-191
Application Western Blot: Use 1/10 as starting working dilution. 
Immuno Assays.

Finctional assays:  JC4 is useful for Inhibition of toxin activity of CNF1. Does not inhibit the toxin activity of CNF2.
In vitro dilutions have to be made according to the amounts of CNF1 or CNF2 to be inactivated.
Background CNF1 and 2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal binding domain, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.
Concentration 0.1 mg/ml
General Readings
  1. Meysick KC, Mills M, O'Brien AD. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect Immun. 2001 Apr;69(4):2066-74. PubMed PMID: 11254559. (Free PMC Article available, 4 images available)
  2. McNichol BA, Rasmussen SB, Carvalho HM, Meysick KC, O'Brien AD. Two domains of cytotoxic necrotizing factor type 1 bind the cellular receptor, laminin receptor precursor protein. Infect Immun. 2007 Nov;75(11):5095-104. Epub 2007 Aug 20. PubMed PMID: 17709415. (Free PMC Article available, 6 images available)
Storage

Store at 2 - 8 °C.
Shelf life: one year from despatch.

Format
Purification:
Protein G
Buffer System:
PBS
Stabilizers:
0.1% BSA
State:
Liquid 0.2 µm filtered Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
E. coli
Specificity
Specificity:
The monoclonal antibody JC4 is specific for Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic Escherichia coli. 
Clone JC4 recognizes an epitope between amino acids 169 to 191 of the N-terminal binding domain. JC4 neutralizes only CNF1.
Gene ID 3990770

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