NBP1-02934 COL3A1 antibody

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Rabbit anti Human, Mammalia COL3A1


Product Description for COL3A1

Rabbit anti Human, Mammalia COL3A1.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for COL3A1

Product Category Primary Antibodies
Quantity 0.05 mg
Synonyms EDS4A antibody, anti-COL3A1 antibody
Presentation Aff - Purified
Reactivity Hu, Mam
Applications E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide corresponding to Collagen Type III from human and bovine placenta
Add. information Research Areas: Cancer
Application ELISA, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, This antibody is useful for the following applications and at the following dilutions: Western Blot 1:5000-1:10000, immunoprecipitation, ELISA 1:5000-1:10000, Immunohistochemistry-Paraffin 25 ug/ml, Western Blot 1:5000-1:10000, immunoprecipitation, ELISA 1:5000-1:10000, Immunohistochemistry-Paraffin 25 ug/ml,
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Concentration 1 mg/ml
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.01% Sodium Azide
Immunogen affinity purified
Aff - Purified
Collagen Type III from human and bovine placenta.
Human, Mammal

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