NB120-3092 Collagen-2 / Collagen-II antibody

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Mouse anti Chicken, Human, Mouse Collagen-2 / Collagen-II 5B2.5


Product Description for Collagen-2 / Collagen-II

Mouse anti Chicken, Human, Mouse Collagen-2 / Collagen-II 5B2.5.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Collagen-2 / Collagen-II

Product Category Primary Antibodies
Quantity 0.5 ml
Synonyms II, primary osteoarthritis spondyloepiphyseal dysplasia congenital
Presentation Purified
Reactivity Chk, Hu, Ms
Applications ICC/IF, WB
Clonality Monoclonal
Clone 5B2.5
Host Mouse
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

A purified preparation of lathyritic type II collagen from embryonic chicken sternum.
Application immunofluorescence, Western Blot, Western Blot and Immunofluorescence: Not suitable for IHC. The optimal dilution for a specific application should be determined by the investigator. , immunofluorescence assay dependent , Western Blot 1-2 ug/ml for 2 hrs at RT
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues. Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Concentration 0.2 mg/ml
General Readings This product has been used in:Mayne R et al. Connect Tissue Res. 31:11-21 (1994).General / background references:Chen Q et al. Type II collagen during cartilage and corneal development: immunohistochemical analysis with an anti-telopeptide antibody. Dev Dyn 196:47-53 (1993). PubMed PMID: 7687475
Storage Store at 4C. Do not freeze.
protein A purified
Buffer System:
0.09% Sodium Azide
protein A purified
GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues consitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic type II collagen with recombinant stromelysin., This is highly specific to type II collagen and shows no cross-reaction with types I, III, IV, V, VI, IX, X, or XI. In Western blotting, 5B2.5 MAb reacts with the TCA fragment of lathyritic type II collagen after digestion with mammalian collagenase. It does not react with pepsin-digested type II collagen.
Chicken, Human, Mouse

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