R1038 Collagen type I antibody

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0.1 mg / €290.00
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Rabbit anti Bovine, Human, Mammalia, Mouse, Rat Collagen type I

R1038

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Product Description for Collagen type I

Rabbit anti Bovine, Human, Mammalia, Mouse, Rat Collagen type I.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Collagen type I

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Presentation Purified
Reactivity Bov, Hu, Mam, Ms, Rt
Applications C, E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Molecular weight 150 kDa (Target)
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02452
Immunogen:
Collagen type I purified from Human and Bovine placenta.
GeneID:
1277
Add. information Note: Collagen type I consists of alpha-1 (139 kDa) and alpha-2 chains (129kDa). Since collagen type I is a triple helix consisting of one alpha-2 chain and two alpha-1 chains, one can expect bands of the dimeric (~270 kDa) and the trimeric form (~400 kDa). Remember that those chains are cross-linked and can't be broken by typical sample denaturation for SDS-PAGE.
Application Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and western blotting for highly sensitive qualitative analysis. Specific researchers have reported that this antibody is also functional by conventional SDS-PAGE Western blot. See References below for additional details.
Recommended Dilutions:
ELISA: 1/5,000-1/50,000. 
Western blot: 1/1,000-1/10,000. 
Immunoprecipitation: 1/100. 
Immunohistochemistry: 1/50-1/200.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization from human and bovine placenta and cartilage have been extensively purified by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS PAGE and immunoblotting.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Villone D, Fritsch A, Koch M, Bruckner-Tuderman L, Hansen U, Bruckner P. Supramolecular interactions in the dermo-epidermal junction zone: anchoring fibril-collagen VII tightly binds to banded collagen fibrils. J Biol Chem. 2008 Sep 5;283(36):24506-13. doi: 10.1074/jbc.M802415200. Epub 2008 Jul 3. PubMed PMID: 18599485. (Free PMC Article available, 9 images available)
  2. Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem. 2012 May 25;287(22):18700-9. doi: 10.1074/jbc.M111.336073. Epub 2012 Apr 9. PubMed PMID: 22493504. (Free PMC Article available, 8 images available)
  3. Hoyas Fernández, JA. Terapia regenerativa del tendón supraespinoso: estudio realizado en un modelo murino de lesión crónica, Thesis 2014.
  4. González-Miguel J, Morchón R, Siles-Lucas M, Simón F. Fibrinolysis and proliferative endarteritis: two related processes in chronic infections? The model of the blood-borne pathogen Dirofilaria immitis. PLoS One. 2015 Apr 13;10(4):e0124445. doi: 10.1371/journal.pone.0124445. eCollection 2015. PubMed PMID: 25875022. (Free PMC Article available, 12 images available)
  5. Nyström A, Thriene K, Mittapalli V, Kern JS, Kiritsi D, Dengjel J, et al. Losartan ameliorates dystrophic epidermolysis bullosa and uncovers new disease mechanisms. EMBO Mol Med. 2015 Jul 20;7(9):1211-28. doi: 10.15252/emmm.201505061. PubMed PMID: 26194911. (Free PMC Article available, 7 images available)
  6. Carriel V, Scionti G, Campos F, Roda O, Castro B, Cornelissen M, et al. In vitro characterization of a nanostructured fibrin agarose bio-artificial nerve substitute. J Tissue Eng Regen Med. 2015 Jul 14. doi: 10.1002/term.2039. PubMed PMID: 26177604.
  7. Caley MP, King H, Shah N, Wang K, Rodriguez-Teja M, Gronau JH, et al. Tumor-associated Endo180 requires stromal-derived LOX to promote metastatic prostate cancer cell migration on human ECM surfaces. Clin Exp Metastasis. 2016 Feb;33(2):151-65. doi: 10.1007/s10585-015-9765-7. Epub 2015 Nov 13. PubMed PMID: 26567111. (Free PMC Article available)
  8. Oleg Tsuprykov2, Ryotaro Ando, Christoph Reichetzeder, Karoline von Websky, Viktoriia Antonenko, Yuliya Sharkovska, Lyubov Chaykovska, Jan Rahnenführer, Ahmed A. Hasan, Harald Tammen, Markus Alter, Thomas Klein, Seiji Ueda, Sho-ichi Yamagishi, Seiya Okuda, Berthold Hocher.The dipeptidyl peptidase inhibitor linagliptin and the angiotensin II receptor blocker telmisartan show renal benefit by different pathways in rats with 5/6 nephrectomy. Kidney International Volume 89, Issue 5, May 2016, Pages 1049–1061. doi:10.1016/j.kint.2016.01.016.
General Readings
  1. Stefanovic B, Schnabl B, Brenner DA. Inhibition of collagen alpha 1(I) expression by the 5' stem-loop as a molecular decoy. J Biol Chem. 2002 May 17;277(20):18229-37. Epub 2002 Mar 11. PubMed PMID: 11889120.
  2. Hashimoto N, Jin H, Liu T, Chensue SW, Phan SH. Bone marrow-derived progenitor cells in pulmonary fibrosis. J Clin Invest. 2004 Jan;113(2):243-52. PubMed PMID: 14722616. (Free PMC Article available, 6 images available)
  3. Hazra S, Xiong S, Wang J, Rippe RA, Krishna V, Chatterjee K, et al. Peroxisome proliferator-activated receptor gamma induces a phenotypic switch from activated to quiescent hepatic stellate cells. J Biol Chem. 2004 Mar 19;279(12):11392-401. Epub 2003 Dec 31. PubMed PMID: 14702344.
  4. She H, Xiong S, Hazra S, Tsukamoto H. Adipogenic transcriptional regulation of hepatic stellate cells. J Biol Chem. 2005 Feb 11;280(6):4959-67. Epub 2004 Nov 9. PubMed PMID: 15537655.
  5. Mak KM, Kwong AJ, Chu E, Hoo NM. (2011) Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology. 5 DEC 2011. DOI: 10.1002/ar.21525.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
For extended storage, mix with an equal volume of glycerol.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) purified Ig fraction
Purified
Specificity
Specificity:
This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
This antibody reacts with most mammalian Type I Collagens and has negligible cross-reactivity with Type II, III, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.
Species:
Human, Mouse, Rat and Bovine.
Gene ID 1277

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type I (10 products)

Catalog No. Species Pres. Purity   Source  

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
0.1 g / €1,390.00
  Acris Antibodies GmbH

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
50 mg / €900.00
  Acris Antibodies GmbH

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
10 mg / €330.00
  Acris Antibodies GmbH

Collagen type I

Collagen type I Human > 98 % (Chromatographically and Immunologically pure). Placenta
0.5 mg / €360.00
  Acris Antibodies GmbH

Collagen type I

Western blot of Human Collagen Type I (GTX27533). 
Lane 1: Human Collagen Type 1. 
Lane 2: None. 
Load: 50 ng per lane. Primary antibody: Collagen Type I antibody at 1:1,000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block in blocking buffer for 30 min at RT. Predicted/Observed size: 139 & 130 kDa, 139 & 130 kDa for Collagen Type I. Other Band(s): Collagen Type I splice variants and isoforms. n/a
0.5 mg / €410.00
  GeneTex Inc.

Collagen type I alpha 1 chain

Collagen type I Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type I alpha 1 chain

Collagen type I Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Collagen type I

Collagen type I Human
1 mg / €640.00
  Abnova Taiwan Corp.

Collagen type I (Atelo-collagen)

Collagen type I Human Purified > 98 % Fibroblasts
0.1 mg / €290.00
  Funakoshi Co., Ltd.

Collagen type I (Atelo-collagen)

Collagen type I Human Purified > 98 % Fibroblasts
0.5 mg / €640.00
  Funakoshi Co., Ltd.

Positive controls for Collagen type I (1 products)

Catalog No. Species Pres. Purity   Source  

COL1A1 overexpression lysate

COL1A1 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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