TA309096 Collagen type I (Propeptide) antibody

Rabbit Anti-Collagen 1, alpha 1 propeptide Antibody

See related secondary antibodies

Search for all "Collagen type I"

0.1 ml / €405.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Amphibian, Fish, Human, Mammalia, Mouse Collagen type I

TA309096

More Views

  • TA309096

Product Description for Collagen type I

Rabbit anti Amphibian, Fish, Human, Mammalia, Mouse Collagen type I.
Properties: (Propeptide)
Presentation: Azide Free
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Paraffin Sections, Western blot / Immunoblot.

Properties for Collagen type I

Product Category Primary Antibodies
Target Category
Quantity 0.1 ml
Synonyms Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2
Presentation Azide Free
Reactivity Amph, Av, Fi, Hu, Mam, Ms
Applications C, ICC/IF, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Europe, USA/Canada
PDF datasheet View Datasheet
Manufacturer OriGene Technologies, Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
P02452
Immunogen:
Peptide from the Human Collagen I Alpha-1 propeptide sequence.
GeneID:
1277
Property Propeptide
Application Western Blot: 1/1000.
Immunohistochemistry: 1/100.
The antibody also works well for Immunohistochemistry on Paraformaldehyde-Fixed Sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90ºC in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20).
Note that in Paraffin Sections of Formaldehyde-Fixed fibrotic mouse lung tissue, it recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Background Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in
the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).
General Readings
  1. Byers PH. Inherited disorders of collagen gene structure and expression. Am J Med Genet. 1989 Sep;34(1):72-80. PubMed PMID: 2683783.
  2. Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, et al. Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J Biol Chem. 2005 May 13;280(19):19259-69. Epub 2005 Feb 22. PubMed PMID: 15728585.
Storage Store the antibody undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography.
Buffer System:
100ul 10 mM Hepes (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
State:
Liquid purified Ig fraction.
Azide Free
Species Reactivity
Species reactivity (expected):
most (if not all) Species of Mammals, Birds, Amphibians and Fishes.
Species reactivity (tested):
Human and Mouse.
Specificity
Specificity:
Specific for the ~180 kDa propeptide portion of the Collagen I alpha 1 polypeptide.

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type I (9 products)

Catalog No. Species Pres. Purity   Source  

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
  OriGene Technologies GmbH

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
  OriGene Technologies GmbH

Collagen type I

Collagen type I Human Purified > 99.0% as determined by SDS-PAGE
  OriGene Technologies GmbH

Collagen type I

Collagen type I Human > 98 % (Chromatographically and Immunologically pure). Placenta
0.5 mg / €400.00
  OriGene Technologies GmbH

Collagen type I alpha 1 chain

Collagen type I Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Collagen type I alpha 1 chain

Collagen type I Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Collagen type I

Collagen type I Human
  Abnova Taiwan Corp.

Collagen type I (Atelo-collagen)

Collagen type I Human Purified > 98 % Fibroblasts
  Funakoshi Co., Ltd.

Collagen type I (Atelo-collagen)

Collagen type I Human Purified > 98 % Fibroblasts
  Funakoshi Co., Ltd.

Positive controls for Collagen type I (1 products)

Catalog No. Species Pres. Purity   Source  

COL1A1 overexpression lysate

COL1A1 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
  • LinkedIn