AF5710 Collagen type II alpha 1 chain antibody

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0.1 mg / €280.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Bovine, Human, Rabbit, Rat Collagen type II alpha 1 chain II-4C11

Product Description for Collagen type II alpha 1 chain

Mouse anti Bovine, Human, Rabbit, Rat Collagen type II alpha 1 chain II-4C11.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for Collagen type II alpha 1 chain

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Alpha-1 type II collagen, COL2A1
Presentation Purified
Reactivity Bov, Hu, Rb, Rt
Applications C, P, WB
Clonality Monoclonal
Clone II-4C11
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02458
GeneID:
1280
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western blot: 2 µg/ml.
Immunohistochemistry on Frozen Sections:
1-10 µg/ml.
Immunohistochemistry on Paraffin Sections: 1-10 µg/ml, Hyaluronidase treatment.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This may result in diminished reactivity of some antibodies with denatured collagen or formalin fixed, paraffin embedded tissues.
Type II collagen is a fibrillar collagen found in cartilage and the vitreous humor of the eye. Collagen type II is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
Concentration 0.5 mg/ml
Protocols Protocol with frozen, ice-cold acetone-fixed sections
The whole procedure is performed at room temperature:
Wash in PBS.
Block endogenous peroxidase.
Wash in PBS
Block with 10% normal goat serum in PBS for 30 min in a humid chamber.
Incubate with primary antibody clone II-4C11 at 1-10 µg/ml for 1h in a humid chamber.
Wash in PBS.
Incubate with secondary antibody for 1h in a humid chamber (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to human and rat).
Wash in PBS.
Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min.
Wash in PBS.
Counterstain with Mayer's hemalum.

Protocol with formalin-fixed, paraffin-embedded sections (proteinase K pre-treatment)
The whole procedure is performed at room temperature.
Deparaffinize and rehydrate tissue section.
Incubate the tissue section with proteinase K for 7 min (DakoCytomation #S3004).
Wash in distilled water.
Block endogenous peroxidase.
Wash in PBS.
Block with 10% normal goat serum in PBS for 30 min in a humid chamber.
Incubate with primary antibody clone II-4C11 at 1-10 µg/ml for 1h in a humid chamber.
Wash in PBS.
Incubate with secondary antibody for 1h in a humid chamber (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to human and rat).
Wash in PBS.
Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12 min.
Wash in PBS.
Counterstain with Mayer's hemalum.
Product Citations
Purchased from Acris:
  1. Mathieu M, Vigier S, Labour MN, Jorgensen C, Belamie E, Noël D. Induction of mesenchymal stem cell differentiation and cartilage formation by cross-linker-free collagen microspheres. Eur Cell Mater. 2014 Sep 2;28:82-96; discussion 96-7. PubMed PMID: 25179212.
  2. Sieker JT, Kunz M, Weißenberger M, Gilbert F, Frey S, Rudert M, et al. Direct bone morphogenetic protein 2 and Indian hedgehog gene transfer for articular cartilage repair using bone marrow coagulates. Osteoarthritis Cartilage. 2015 Mar;23(3):433-42. doi: 10.1016/j.joca.2014.11.008. Epub 2014 Nov 13. PubMed PMID: 25463442.
  3. Guo G, Lu L, Ji H, Ma Y, Dong R, Tu J, et al. Low intensity pulse ultrasound stimulate chondrocytes growth in a 3-D alginate scaffold through improved porosity and permeability. Ultrasonics. 2015 Apr;58:43-52. doi: 10.1016/j.ultras.2014.12.001. Epub 2014 Dec 16. PubMed PMID: 25543661.
  4. Yan LP, Silva-Correia J, Oliveira MB, Vilela C, Pereira H, Sousa RA, et al. Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance. Acta Biomater. 2015 Jan;12:227-41. doi: 10.1016/j.actbio.2014.10.021. Epub 2014 Oct 23. PubMed PMID: 25449920.
  5. Kleinhans C, Mohan RR, Vacun G, Schwarz T, Haller B, Sun Y, et al. A perfusion bioreactor system efficiently generates cell-loaded bone substitute materials for addressing critical size bone defects. Biotechnol J. 2015 May 26. doi: 10.1002/biot.201400813. PubMed PMID: 26011163.
  6. Bleil J, Sieper J, Maier R, Schlichting U, Hempfing A, Syrbe U, et al. Cartilage in facet joints of patients with ankylosing spondylitis (AS) shows signs of cartilage degeneration rather than chondrocyte hypertrophy: implications for joint remodeling in AS. Arthritis Res Ther. 2015 Jul 17;17:170. doi: 10.1186/s13075-015-0675-5. PubMed PMID: 26123554. (Free PMC Article available, 5 images available)
  7. Lindsey Marie Ott. In Vitro and In Vivo Characterization of Tunable Fibrous Scaffolds for Tracheal Tissue Engineering. Thesis 2008. https://kuscholarworks.ku.edu/bitstream/handle/1808/18378/Ott_ku_0099D_13793_DATA_1.pdf?sequence=1
General Readings
  1. Burgeson, R.E. and Hollister, D.W. Collagen heterogeneity in human cartilage: Identification of several new collagen chains. Biochem. Biophys. Res. Commun., 87. 1124-1131, 1979.
  2. Kumagai J, Sarkar K, Uhthoff HK, Okawara Y, Ooshima A. Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion. J Anat. 1994 Oct;185 ( Pt 2):279-84. PubMed PMID: 7961134. (Free PMC Article available)
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Stabilizers:
0.5% BSA (protease-free)
State:
Liquid purified Ig fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rabbit, Bovine, and Rat.
Specificity
Specificity:
The antibody specifically reacts with Collagen type II from Human costal cartilage.
Gene ID 1280

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type II alpha 1 chain (7 products)

Catalog No. Species Pres. Purity   Source  

Collagen type II alpha 1 chain (Adult Knee Cartilage)

Collagen type II alpha 1 chain Human > 98 % Chromatographically and immunologically pure. Cartilage
0.1 mg / €640.00
  Acris Antibodies GmbH

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain n/a
50 µg / €430.00
  GeneTex Inc.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
1 mg / €640.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
1 mg / €640.00
  Abnova Taiwan Corp.
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