R1039 Collagen type II alpha 1 chain antibody

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0.1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Bovine, Human, Mouse, Rat, Sheep Collagen type II alpha 1 chain

Product Description for Collagen type II alpha 1 chain

Rabbit anti Bovine, Human, Mouse, Rat, Sheep Collagen type II alpha 1 chain.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Collagen type II alpha 1 chain

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms Alpha-1 type II collagen, COL2A1
Presentation Aff - Purified
Reactivity Bov, Hu, Ms, Rt, Sh
Applications C, E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02458
Immunogen:
Collagen type II purified from Human knee and Bovine nasal cartilage.
GeneID:
1280
Application Anti-Collagen antibodies have been used for Indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for native (non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitive qualitative analysis.
Recommended Dilutions:
ELISA: 1/5,000-1/50,000. 
Western Blot: 1/1,000-1/10,000.
Immunoprecipitation: 1/100.
Immunohistochemistry: 1/50-1/400. 
QC: This product was assayed by Immunoblot and found to be reactive against Collagen II at a dilution of 1/5,000 to 1/10,000.
This product was also assayed against 1.0 µg of Collagen II in a standard Sandwich ELISA using Peroxidase conjugated affinity purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock concentration showed best results.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. For immunization collagens from human and bovine placenta and cartilage extensively purified by limited pepsin digestion and selective salt precipitation were used. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Purchased from Acris:
  1. Jagielski M, Wolf J, Marzahn U, Völker A, Lemke M, Meier C, et al. The influence of IL-10 and TNFα on chondrogenesis of human mesenchymal stromal cells in three-dimensional cultures. Int J Mol Sci. 2014 Sep 9;15(9):15821-44. doi: 10.3390/ijms150915821. PubMed PMID: 25207597. (Free PMC Article available, 9 images available)
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
For extended storage, mix with an equal volume of glycerol.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) purified Ig fraction
Aff - Purified
Specificity
Specificity:
This product has been prepared by Immunoaffinity Chromatography using immobilised antigens followed by extensive cross-adsorbtion against other collagens, Human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Crossreactivity < 1% against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Species:
Human, Mouse, Rat, Bovine, Sheep.
Gene ID 1280

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type II alpha 1 chain (7 products)

Catalog No. Species Pres. Purity   Source  

Collagen type II alpha 1 chain (Adult Knee Cartilage)

Collagen type II alpha 1 chain Human > 98 % Chromatographically and immunologically pure. Cartilage
0.1 mg / €640.00
  Acris Antibodies GmbH

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain n/a
50 µg / €430.00
  GeneTex Inc.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
0.1 mg / €240.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
1 mg / €640.00
  Abnova Taiwan Corp.

Collagen type II alpha 1 chain

Collagen type II alpha 1 chain Human
1 mg / €640.00
  Abnova Taiwan Corp.
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