AF5810 Collagen type III alpha 1 chain antibody

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0.1 mg / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Rabbit Collagen type III alpha 1 chain III-53

Product Description for Collagen type III alpha 1 chain

Mouse anti Human, Rabbit Collagen type III alpha 1 chain III-53.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for Collagen type III alpha 1 chain

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms COL3A1
Presentation Purified
Reactivity Hu, Rb
Applications C, P, WB
Clonality Monoclonal
Clone III-53
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02461
Immunogen:
Human placenta.
GeneID:
1281
Add. information Customer feedback/publication describes reactivity with dog (see Rortveit, 2012 for reference).
Application Western blot: 2 µg/ml.
Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml, Paraffin sections may require heat mediated antigen retrieval with 10mM citrate buffer pH 6.0; According to customer information this antibody can be used on decalcified tissue pretreated with 0.2% hyaluronidase in sodium acetate buffer for 1h at 37°C.
Background Collagen III is a fibrillar collagen that is found in extensible connective tissues such as skin, lung, and the vascular system, frequently in association with type I Collagen. Mutations in the gene are associated with Ehlers Danlos syndrome type IV, and with aortic and arterial aneurysms. Although alternate transcripts have been detected for this gene, they are the result of mutations; these mutations alter splicing, often leading to the exclusion of multiple exons.
Concentration 0.5 mg/ml
Product Citations
Purchased from Acris:
  1. Rørtveit R, Lingaas F, Bønsdorff T, Eggertsdóttir AV, Grøndahl AM, Thomassen R, et al. A canine autosomal recessive model of collagen type III glomerulopathy. Lab Invest. 2012 Oct;92(10):1483-91. doi: 10.1038/labinvest.2012.112. Epub 2012 Aug 13. PubMed PMID: 22890554.
  2. Koulikovska M, Rafat M, Petrovski G, Veréb Z, Akhtar S, Fagerholm P, et al. Enhanced Regeneration of Corneal Tissue Via a Bioengineered Collagen Construct Implanted by a Nondisruptive Surgical Technique. Tissue Eng Part A. 2014 Nov 20. PubMed PMID: 25412075.
  3. Karpus ON, Kiener HP, Niederreiter B, Yilmaz-Elis A, van der Kaa J, Ramaglia V, et al. CD55 deposited on synovial collagen fibers protects from immune complex-mediated arthritis. Arthritis Res Ther. 2015 Jan 17;17(1):6. PubMed PMID: 25596646. (Free PMC Article available, 5 images available)
General Readings
  1. Mayne, R. et al. Isolation and partial characterization of basement membrane-link collagens from bovine thoracic aorta. Artery, 7. 262-280, 1980
  2. Muragaki, Y. et al., Experimental study on alteration of collagen metabolism in liver fibrosis. J. Wakayama Med. Soc., 36. 195-208, 1985 (in Japanese)
  3. Van der Loos, C. M. et al., Histrochemical J., 26, 347-354, 1994.
  4. Kumagai J, Sarkar K, Uhthoff HK, Okawara Y, Ooshima A. Immunohistochemical distribution of type I, II and III collagens in the rabbit supraspinatus tendon insertion. J Anat. 1994 Oct;185 ( Pt 2):279-84. PubMed PMID: 7961134. (Free PMC Article available)
  5. Marijianowski MM, Teeling P, Mann J, Becker AE. Dilated cardiomyopathy is associated with an increase in the type I/type III collagen ratio: a quantitative assessment. J Am Coll Cardiol. 1995 May;25(6):1263-72. PubMed PMID: 7722119.
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A.
Buffer System:
0.1 M Na-Phosphate buffer, pH 7.0
Stabilizers:
0.5% BSA (Protease-free)
State:
Liquid purified Ig fraction.
Purified
Species Reactivity
Species reactivity (tested):
Human and Rabbit.
Specificity
Specificity:
The antibody specifically reacts with Collagen III.
Gene ID 1281

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type III alpha 1 chain (11 products)

Catalog No. Species Pres. Purity   Source  

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
0.1 g / €1,390.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
50 mg / €900.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
10 mg / €330.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain (Native)

Collagen type III alpha 1 chain Rat > 90 % pure by SDS-PAGE.
Preparation: Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment.
Purification: Differential Salt Precipitation.
Rat Collagen type III > 90%
Rat collagen type I 10%
Rat collagen (other types) < 1%
Non-collagenous proteins < 0.5%
Tail
10 mg / €220.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human > 98 % Chromatographically and immunologically pure. Placenta
0.5 mg / €360.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Western Blot of Collagen III 
Lane 1: Human Collagen III 
Load: 100 ng per lane Primary antibody: Collagen III Antibody (GTX27535) at 1:1000 o/n at 4°C Secondary antibody: DyLight™ 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa n/a
0.5 mg / €410.00
  GeneTex Inc.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified
25 µg / €510.00
  Abnova Taiwan Corp.

Positive controls for Collagen type III alpha 1 chain (1 products)

Catalog No. Species Pres. Purity   Source  

COL3A1 overexpression lysate

COL3A1 overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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