R1040 Collagen type III alpha 1 chain antibody

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0.1 mg / €290.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Bovine, Human Collagen type III alpha 1 chain

R1040

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Product Description for Collagen type III alpha 1 chain

Rabbit anti Bovine, Human Collagen type III alpha 1 chain.
Presentation: Aff - Purified
Product is tested for Frozen Sections, Paraffin Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for Collagen type III alpha 1 chain

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms COL3A1
Presentation Aff - Purified
Reactivity Bov, Hu
Applications C, E, IP, P, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02461
Immunogen:
Collagen type III purified from Human and Bovine placenta
GeneID:
1281
Application Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for native (non-denaturating, non-dissociating) PAGE and Western blotting for highly sensitive qualitative analysis.
Recommended Dilutions:
ELISA: 1/5,000-1/50,000.
Western blot: 1/1,000-1/10,000.
Immunoprecipitation: 1/100.
Immunohistochemistry: 1/50-1/200.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of type-specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens for immunization are purified from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation.
This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type'-specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Product Citations
Originator or purchased from resellers:
  1. Mak KM, Kwong AJ, Chu E, Hoo NM. (2011) Hepatic Steatosis, Fibrosis, and Cancer in Elderly Cadavers. Cancer Biology. 5 DEC 2011. DOI: 10.1002/ar.21525.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
For extended storage, mix with an equal volume of glycerol.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Immunoaffinity Chromatography
Buffer System:
0.125 M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0
Preservatives:
0.01% (w/v) Sodium Azide
Stabilizers:
None
State:
Liquid (sterile filtered) purified Ig fraction
Aff - Purified
Specificity
Specificity:
This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
Typically less than 1% cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.
This antibody reacts with most mammalian Type III Collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens.
Non-specific cross reaction of anti-Collagen antibodies with other Human serum proteins or non-collagen extracellular matrix proteins is negligible.
Species:
Human and Bovine.
Gene ID 1281

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type III alpha 1 chain (11 products)

Catalog No. Species Pres. Purity   Source  

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
0.1 g / €1,390.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
50 mg / €900.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified > 95.0% as determined by SDS-PAGE
10 mg / €330.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain (Native)

Collagen type III alpha 1 chain Rat > 90 % pure by SDS-PAGE.
Preparation: Collagen was extracted from washed dissected tissue into dilute acetic acid after pepsin treatment.
Purification: Differential Salt Precipitation.
Rat Collagen type III > 90%
Rat collagen type I 10%
Rat collagen (other types) < 1%
Non-collagenous proteins < 0.5%
Tail
10 mg / €220.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human > 98 % Chromatographically and immunologically pure. Placenta
0.5 mg / €360.00
  Acris Antibodies GmbH

Collagen type III alpha 1 chain

Western Blot of Collagen III 
Lane 1: Human Collagen III 
Load: 100 ng per lane Primary antibody: Collagen III Antibody (GTX27535) at 1:1000 o/n at 4°C Secondary antibody: DyLight™ 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block for 30 min at RT Predicted/Observed size: 138 kDa, 138 kDa n/a
0.5 mg / €410.00
  GeneTex Inc.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

Collagen type III alpha 1 chain

Collagen type III alpha 1 chain Human Purified
25 µg / €510.00
  Abnova Taiwan Corp.

Positive controls for Collagen type III alpha 1 chain (1 products)

Catalog No. Species Pres. Purity   Source  

COL3A1 overexpression lysate

COL3A1 overexpression lysate
0.1 mg / €480.00
  OriGene Technologies, Inc.
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