AF5910 Collagen type IV antibody

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Mouse anti Human Collagen type IV IV-4H12


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  • AF5910

Product Description for Collagen type IV

Mouse anti Human Collagen type IV IV-4H12.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for Collagen type IV

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms COL4A1
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone IV-4H12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Swiss Prot Num:
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application ELISA: Reported in Ref.4
Western blot: 
10 μg/ml (See also Ref.3)
Immunohistochemistry on Frozen Sections: 1-10 µg/ml.
Immunohistochemistry on Paraffin Sections:
 1-10 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Collagen IV is a major constituent of the basement membranes along with laminins, proteoglycans and enactins. It is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. It can form insoluble fibers with high tensile strength. Collagen IV is useful in detecting the loss of parts of basement membranes in carcinomas.
Concentration 0.5 mg/ml
Product Citations
Purchased from Acris:
  1. Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, et al. The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens. J Biol Chem. 2012 May 25;287(22):18700-9. doi: 10.1074/jbc.M111.336073. Epub 2012 Apr 9. PubMed PMID: 22493504. (Free PMC Article available, 8 images available)
  2. Flenkenthaler F, Windschüttl S, Fröhlich T, Schwarzer JU, Mayerhofer A, Arnold GJ. Secretome analysis of testicular peritubular cells: a window into the human testicular microenvironment and the spermatogonial stem cell niche in man. J Proteome Res. 2014 Mar 7;13(3):1259-69. doi: 10.1021/pr400769z. Epub 2014 Jan 30. PubMed PMID: 24422521.
General Readings 1. Mayne R, Zettergren JG, Mayne PM, Bedwell NW. Isolation and partial characterization of basement membrane-like collagens from bovine thoracic aorta. Artery. 1980;7(4):262-80. PubMed PMID: 7213017.
2. Matsumoto, E. et al. Foreknowledge of liver fibrosis: development of immunoassay system for serum collagen peptides with monoclonal antibody. J. Wakayaka Med. Soc., 39. 87-106, 1988 (In Japanese). 
3. Matsumoto, E. et al. Acta pathol. Jpn., 39, 217-223, 1989. 
4. Obata, K. et al., One step sandwich enzyme immunoassay for human type IVcollagen using monoclonal antibodies. Clin. Clim. Acta, 181, 293-304, 1989.

Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
0.1 M Sodium Phosphate buffer, pH 7.0
0.5% BSA (protease-free)
Liquid purified Ig fraction
Species Reactivity
Species reactivity (tested):
The antibody specifically reacts with the 7S domain of Human Collagen Type IV. 

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type IV (1 products)

Catalog No. Species Pres. Purity   Source  

Collagen type IV

Collagen type IV Human Chromatographically and Immunologically pure. Placenta
0.5 mg / €450.00
  OriGene Technologies GmbH
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