AF6210 Collagen type VI antibody

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0.1 mg / €190.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human, Rabbit Collagen type VI VI-26

Product Description for Collagen type VI

Mouse anti Human, Rabbit Collagen type VI VI-26.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Western blot / Immunoblot.

Properties for Collagen type VI

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms COL6A1
Presentation Purified
Reactivity Hu, Rb
Applications C, P, WB
Clonality Monoclonal
Clone VI-26
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P12109
GeneID:
1291
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Western blot: 10 µg/ml.
Immunohistochemistry on Frozen and Paraffin Sections: 1-10 µg/ml. 
Heat induced antigen retrieval with citrat buffer, pH 6.2 using a pressure cooker was preformed. Sections were blocking using a commercially available casein solution. Signal was generated using a commercially available polymer HRP detection system and DAB.
Background Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.
Concentration 0.5 mg/ml
General Readings
  1. Mayne R, Zettergren JG, Mayne PM, Bedwell NW. Isolation and partial characterization of basement membrane-like collagens from bovine thoracic aorta. Artery. 1980;7(4):262-80. PubMed PMID: 7213017.
  2. Hessle, H. and Engvall, E. type VI Collagen. Sudies on its localization, structure and biosynthetic form with monoclonal antibodies. J. Biol. Chem., 259. 3955-3961, 1984.
  3. Muragaki, Y. et al. Experimental study on alteration of collagen metabolism in liver fibrosis. J. Wakayama Med. Soc., 36. 195-208, 1985 (In Japanese).
Storage Upon receipt, store undiluted (in aliquots) at -20°C.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
0.1M Sodium Phosphate buffer, pH 7.0
Stabilizers:
0.5% Protease-free BSA
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human and Rabbit.
Specificity
Specificity:
This antibody specifically reacts with hCL(VI). Especially it recognizes tertialy structure. It doesn't recognize Collagen VI without the structure.
Gene ID 1291

Accessory Products

Proteins and/or Positive Controls

Proteins for Collagen type VI (10 products)

Catalog No. Species Pres. Purity   Source  

Collagen type VI

Collagen type VI Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Collagen type VI

Collagen type VI Human Purified E. coli
10 µg / €630.00
  Acris Antibodies GmbH

Collagen type VI

Collagen type VI Human Purified E. coli
5 µg / €400.00
  Acris Antibodies GmbH

Collagen type VI

Collagen type VI Human Purified E. coli
2 µg / €310.00
  Acris Antibodies GmbH

Collagen type VI

Collagen type VI Human Chromatographically and Immunologically pure. Placenta
0.5 mg / €360.00
  Acris Antibodies GmbH

Collagen type VI

Collagen type VI Bovine > 98 % pure. Prepared from Bovine Placenta and is chromatographically and immunologically pure. Placenta
0.5 mg / €330.00
  Acris Antibodies GmbH

Collagen type VI

Collagen type VI n/a
0.5 mg / €410.00
  GeneTex Inc.

Collagen type VI

Collagen type VI n/a
0.5 mg / €410.00
  GeneTex Inc.

Collagen type VI

Collagen type VI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Collagen type VI alpha 1 chain

Collagen type VI Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Positive controls for Collagen type VI (1 products)

Catalog No. Species Pres. Purity   Source  

COL6A1 overexpression lysate

COL6A1 overexpression lysate
0.1 mg / €280.00
  OriGene Technologies, Inc.
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