AM01331PU-N Complement SC5b-9 (TCC / MAC) antibody

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0.1 mg / €480.00
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Mouse anti Human Complement SC5b-9 (TCC / MAC) 3R2/0

AM01331PU-N

Product Description for Complement SC5b-9 (TCC / MAC)

Mouse anti Human Complement SC5b-9 (TCC / MAC) 3R2/0.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for Complement SC5b-9 (TCC / MAC)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Membrane attack complex, Terminal complement complex
Presentation Purified
Reactivity Hu
Applications C, E, F, WB
Clonality Monoclonal
Clone 3R2/0
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control AM03110PU-N, SM12P
Add. information Removal of Sodium Azide is recommended prior to use in functional assays.
Application ELISA.
Western Blot.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.

Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Background Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.
Concentration 1.0 mg/ml
General Readings
  1. Biesecker G. The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor. J Immunol. 1990 Jul 1;145(1):209-14. PubMed PMID: 1694202.
  2. Greenstein JD, Peake PW, Charlesworth JA. The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation. Clin Exp Immunol. 1995 Apr;100(1):40-6. PubMed PMID: 7697921. (Free PMC Article available)
  3. Rus H, Cudrici C, Niculescu F. C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection. Ann Med. 2005;37(2):97-104. PubMed PMID: 16026117.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Borate buffered saline, pH8.4 containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody recognises the SC5b-9 complex of around 330 kDa.
Species:
Human.

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