AM01331PU-N Complement SC5b-9 (TCC / MAC) antibody

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0.1 mg / €480.00
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Mouse anti Human Complement SC5b-9 (TCC / MAC) 3R2/0

AM01331PU-N

Product Description for Complement SC5b-9 (TCC / MAC)

Mouse anti Human Complement SC5b-9 (TCC / MAC) 3R2/0.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot, Flow Cytometry.

Properties for Complement SC5b-9 (TCC / MAC)

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Membrane attack complex, Terminal complement complex
Presentation Purified
Reactivity Hu
Applications C, E, F, WB
Clonality Monoclonal
Clone 3R2/0
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Add. information Removal of Sodium Azide is recommended prior to use in functional assays.
Application ELISA.
Western Blot.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.

Recommended Positive Control: Kidney from post streptoccal glomerulonephritis patients.
Background Both the classical and alternative complement pathways result in the formation of the cytolysis inducing C5b-9 complex. This complex is composed of 190 kDa C5b which is bound to cells via 71 kDa C9. Sublytic assembly of C5b-9 on plasma membranes induces cell cycle activation and survival. The binding of C5b-9 to the 75 kDa S-protein (or vitronectin) in the fluid phase prevents C5b-9 from assembling on the plasma membrane, deactivating it and forming the SC5b-9 complex. SC5b-9 is stable in vitro and is therefore a reliable indicator of terminal complement pathway activation.
Concentration 1.0 mg/ml
General Readings
  1. Biesecker G. The complement SC5b-9 complex mediates cell adhesion through a vitronectin receptor. J Immunol. 1990 Jul 1;145(1):209-14. PubMed PMID: 1694202.
  2. Greenstein JD, Peake PW, Charlesworth JA. The kinetics and distribution of C9 and SC5b-9 in vivo: effects of complement activation. Clin Exp Immunol. 1995 Apr;100(1):40-6. PubMed PMID: 7697921. (Free PMC Article available)
  3. Rus H, Cudrici C, Niculescu F. C5b-9 complement complex in autoimmune demyelination and multiple sclerosis: dual role in neuroinflammation and neuroprotection. Ann Med. 2005;37(2):97-104. PubMed PMID: 16026117.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Borate buffered saline, pH8.4 containing 0.09% Sodium Azide as preservative
State:
Liquid purified IgG fraction
Purified
Specificity
Specificity:
This antibody recognises the SC5b-9 complex of around 330 kDa.
Species:
Human.

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