discontinued

NSB854 Cortactin phospho antibody

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Rabbit anti Mouse Cortactin phospho

Product Description for Cortactin phospho

Rabbit anti Mouse Cortactin phospho.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for Cortactin phospho

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Ms
Applications ICC/IF, WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide (Mouse) derived from the region of mouse cortactin that contains tyrosine 421.
Application Immunocytochemistry, Western Blot, ICC: Use at an assay dependant dilution. WB: Predicted molecular weight: 83 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user., Western Blot 0.1 - 1.0 ug/ml, Immunocytochemistry
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Concentration 0.35 mg/ml
General Readings General / background references:Lopez I et al. Thrombopoietin stimulates cortactin translocation to the cytoskeleton independently of tyrosine phosphorylation. Biochem J 356:875-81 (2001). PubMed PMID: 11389697
Li Y et al. Tyrosine phosphorylation of cortactin is required for H2O2-mediated injury of human endothelial cells. J Biol Chem 275:37187-93 (2000). PubMed PMID: 10952984
Kapus A et al. Cell volume-dependent phosphorylation of proteins of the cortical cytoskeleton and cell-cell contact sites. The role of Fyn and FER kinases. J Biol Chem 275:32289-98 (2000). PubMed PMID: 10921917
Liu J et al. Src is required for cell migration and shape changes induced by fibroblast growth factor 1. Oncogene 18:6700-6 (1999). PubMed PMID: 10597276
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
Cortactin phospho [Tyr421]
Species:
Human (-), Mouse

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