discontinued

NSB856 Cortactin phospho antibody

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0.1 ml / €380.00

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Rabbit anti Mouse Cortactin phospho

Product Description for Cortactin phospho

Rabbit anti Mouse Cortactin phospho.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Cortactin phospho

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Immunogen:
Synthetic peptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence).
Application Western Blot, Western Blot Predicted molecular weight 83 kDa. , Western Blot 0.1 - 1.0 ug/ml
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Concentration 0.35 mg/ml
General Readings General / background references:Dudek SM et al. Novel interaction of cortactin with endothelial cell myosin light chain kinase. Biochem Biophys Res Commun 298:511-9 (2002). PubMed PMID: 12408982
Arderiu G et al. Primary arrest of circulating platelets on collagen involves phosphorylation of Syk, cortactin and focal adhesion kinase: studies under flow conditions. Biochem J 364:65-71 (2002). PubMed PMID: 11988077
Weed SA & Parsons JT Cortactin: coupling membrane dynamics to cortical actin assembly. Oncogene 20:6418-34 (2001). PubMed PMID: 11607842
Kapus A et al. Cell volume-dependent phosphorylation of proteins of the cortical cytoskeleton and cell-cell contact sites. The role of Fyn and FER kinases. J Biol Chem 275:32289-98 (2000). PubMed PMID: 10921917
5. Weed, S.A., et al. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. J. Cell Biol. 151(1): 29-40, 2000.
6. Huang, C., et al. The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells. 273(40): 25770-25776, 1998.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Format
Purification:
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Purity:
Immunogen affinity purified
State:
Aff - Purified
Specificity
Specificity:
Cortactin Phosphospecific [Tyr466]
Species:
Mouse

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