NSB856 Cortactin phospho antibody

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0.1 ml / €380.00

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Rabbit anti Mouse Cortactin phospho

Product Description for Cortactin phospho

Rabbit anti Mouse Cortactin phospho.
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Cortactin phospho

Product Category Primary Antibodies
Quantity 0.1 ml
Presentation Aff - Purified
Reactivity Ms
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype IgG
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Synthetic peptide derived from the region of mouse cortactin that contains tyrosine 466 (tyrosine 470 in the human sequence).
Application Western Blot, Western Blot Predicted molecular weight 83 kDa. , Western Blot 0.1 - 1.0 ug/ml
Background Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.
Concentration 0.35 mg/ml
General Readings General / background references:Dudek SM et al. Novel interaction of cortactin with endothelial cell myosin light chain kinase. Biochem Biophys Res Commun 298:511-9 (2002). PubMed PMID: 12408982
Arderiu G et al. Primary arrest of circulating platelets on collagen involves phosphorylation of Syk, cortactin and focal adhesion kinase: studies under flow conditions. Biochem J 364:65-71 (2002). PubMed PMID: 11988077
Weed SA & Parsons JT Cortactin: coupling membrane dynamics to cortical actin assembly. Oncogene 20:6418-34 (2001). PubMed PMID: 11607842
Kapus A et al. Cell volume-dependent phosphorylation of proteins of the cortical cytoskeleton and cell-cell contact sites. The role of Fyn and FER kinases. J Biol Chem 275:32289-98 (2000). PubMed PMID: 10921917
5. Weed, S.A., et al. Cortactin localization to sites of actin assembly in lamellipodia requires interactions with F-actin and the Arp2/3 complex. J. Cell Biol. 151(1): 29-40, 2000.
6. Huang, C., et al. The role of tyrosine phosphorylation of cortactin in the locomotion of endothelial cells. 273(40): 25770-25776, 1998.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Immunogen affinity purified
Buffer System:
0.05% Sodium Azide
Immunogen affinity purified
Aff - Purified
Cortactin Phosphospecific [Tyr466]

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