discontinued

BP396 Cortisol Binding Globulin (CBG) antibody

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Rabbit anti Human Cortisol Binding Globulin (CBG)

BP396

Product Description for Cortisol Binding Globulin (CBG)

Rabbit anti Human Cortisol Binding Globulin (CBG).
Presentation: Purified
Product is tested for Radioimmunoassay.

Properties for Cortisol Binding Globulin (CBG)

Product Category Primary Antibodies
Quantity 0.2 ml
Synonyms Hydrocortisone
Presentation Purified
Reactivity Hu
Applications R
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Native human CBG
Application RIA (I125): use a 1/500 dilution
Assay buffer: 0.02 M phosphate buffer, pH 7.4, containing 0.02% sodium azide, 9% NaCl and 0.5% BSA.
Sensitivity: 0.8 ng/tube.
Protocols RIA PROTOCOL
To each tube add:
200 µl phosphate buffer
100 µl antibody
100 µl standard or sample
100 µl tracer (ca. 20,000 cpm)
Incubation: 3 hours at 37°C
Separation of the free hormone and the bound hormone by double antibody method.
Add 500 µl of anti-rabbit precipitating antibody on insoluble protein, incubate for 30 minutes, add 2 ml phosphate buffer, mix, centrifuge 15 minutes at 2000 - 2500 g, aspirate and count.
NOTE:
The final dilution described should give around 30% binding of the total tracer (B0/T).
The titre and the sensitivity of the RIA are dependent on the particular experimental procedure as well as on the quality of the tracer.
Storage Aliquot and store at -20°C.
Shelf life: one year from despatch.
Format
Purification:
Blue Sepharose chromatography
Buffer System:
PBS, pH 7.2, containing 50% glycerol
State:
Liquid Ig fraction
Purified
Specificity
Specificity:
The antibody recognizes CBG.
Cross reactivities
TeBG: 0.7%
TBG, RBP, Albumin: 0%
Species:
Human.

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