AP51139PU-N Cullin 1 (C-term) antibody

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0.4 ml / €370.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human Cullin 1

Product Description for Cullin 1

Rabbit anti Human Cullin 1.
Properties: (C-term)
Presentation: Aff - Purified
Product is tested for Western blot / Immunoblot.

Properties for Cullin 1

Product Category Primary Antibodies
Target Category
Quantity 0.4 ml
Synonyms CUL-1, CUL1, Cullin-1
Presentation Aff - Purified
Reactivity Hu
Applications WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Molecular weight 89679 Da
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q13616
Immunogen:
KLH conjugated synthetic peptide between 679-708 amino acids from the C-terminal region of human CUL1
GeneID:
8454
Property C-term
Application ELISA: 1/1000.
Western Blot: 1/100 - 1/500.
Background Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF(SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquination of ORC1L, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(Cyclin F) directs ubiquitination of CP110 (By similarity).
Concentration lot specific
General Readings
  1. Gao, D., et al. Mol. Cell 39(5):797-808(2010)
  2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
  3. Kleiger, G., et al. Cell 139(5):957-968(2009)
  4. Cunningham, J.M., et al. Br. J. Cancer 101(8):1461-1468(2009)
  5. Korzeniewski, N., et al. Cancer Res. 69(16):6668-6675(2009)
Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Affinity chromatography on Protein A
Buffer System:
PBS
Preservatives:
0.09% (W/V) sodium azide
State:
Liquid purified Ig fraction
Aff - Purified
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This antibody reacts to CUL1.
Gene ID 8454

Accessory Products

Proteins and/or Positive Controls

Proteins for Cullin 1 (7 products)

Catalog No. Species Pres. Purity   Source  

Cullin 1

Cullin 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Cullin 1 (1-410, His-tag)

Recombinant human CUL1, 1- 410 aa, His-tagged Human Purified > 95 % E. coli
0.25 mg / €750.00
  OriGene Technologies GmbH

Cullin 1 (1-410, His-tag)

Recombinant human CUL1, 1- 410 aa, His-tagged Human Purified > 95 % E. coli
50 µg / €300.00
  OriGene Technologies GmbH

Cullin 1

Cullin 1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cullin 1

Cullin 1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Cullin 1

Cullin 1 Human Purified
  Novus Biologicals Inc.

Cullin 1

Cullin 1 Human Purified
  Novus Biologicals Inc.

Positive controls for Cullin 1 (1 products)

Catalog No. Species Pres. Purity   Source  

Cullin 1 Lysate

Western Blot: Cullin 1 Lysate [NBL1-09606] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for CUL1
  Novus Biologicals Inc.
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