BM2283 Cytokeratin 1+2+10+11 antibody

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Mouse anti Human Cytokeratin 1+2+10+11 AE2


Product Description for Cytokeratin 1+2+10+11

Mouse anti Human Cytokeratin 1+2+10+11 AE2.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 1+2+10+11

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone AE2
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human epidermal keratin
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry on frozen and paraffin embedded sections: 1/50; with paraffin-embedded sections, protease pretreatment is required prior to antibody application. Incubation time: 1h at RT; extended with paraffin-embedded sections (over night at 2 - 8°C). Immunofluorescence. Western Blot. Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
General Readings Moll, R., Franke, W.W., Schiller, D.L., Geiger, B. and Krepler, R.: The catalog of human cytokeratins: Patterns of expression in normal epithelia, tumors and cultured cells. Cell 31, 11-24 (1982)
Shi, S.-R., Goodman, M.L., Chan, A.K., Pilch, B.Z., Chen, L.B. and Sun, T.-T.: Immunohistochemical study of nasopharyngeal carcinoma using monoclonal anti-keratin antibodies. Am. J. Pathol. 117, 53-63 (1984)
Sun, T.-T., Eichner, R., Cooper, D., Schermer, A., Nelson, W.G. and Weiss, R.A.: Classification, expression and possible mechanisms of evolution of mammalian epithelial keratins: A unifying model. The Cancer Cell 1, 169-176 (1984)
Tseng, S.C.G., Jarvinen, M., Nelson, W.G., Huang, J.-W., Woodcock-Mitchell, J. and Sun, T.-T.: Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30, 361-372 (1982)
Tseng, S.C., Hatchell, D., Tierney, N., Huang, A.J.-W. and Sun, T.-T.: Expression of specific keratin markers by rabbit corneal, conjunctival and esophageal epithelia during vitamin A deficiency-induced keratinization. J. Cell Biol. 99, 2279-2286 (1984)
Woodcock-Mitchell, J., Eichner, R., Nelson, W.G. and Sun, T.-T.: Immunolocalization of keratin polypeptides in human epidermis using monoclonal an
Storage Store the antibody at 2-8°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
AE 2 represents an excellent marker for skin-type differentiation. Reacts with acidic 56.5 kD (nos. 10 and 11) and basic 65-67 kD (nos. 1 and 2) cytokeratins immunolocalized in the suprabasal layers of human epidermis. These keratins are also found in corneal, conjunctival , and oesophageal epithelia when keratinization is observed during vitamin A deficiency. Cross-reaction with filaggrin, a keratin-associated protein believed to play a role in keratin-keratin aggregations.

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