BM5047P Cytokeratin 13 antibody

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0.1 mg / €340.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human, Rat, Zebrafish Cytokeratin 13 1C7

Product Description for Cytokeratin 13

Mouse anti Human, Rat, Zebrafish Cytokeratin 13 1C7.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Western blot / Immunoblot.

Properties for Cytokeratin 13

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13
Presentation Purified
Reactivity Hu, Rt, Ze
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone 1C7
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13646
Immunogen:
Cytokeratin preparation from Human esophagus.
GeneID:
3860
Isotype control AM03096PU-N
Application Immunofluorescence.
Western blot:
 1/100-1/1000. 
Immunohistochemistry on Frozen sections: 1/25-1/200 ABC as detection reagent.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9-7.8.
The individual human cytokeratins are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings
  1. van Muijen GN, Ruiter DJ, Franke WW, Achtstätter T, Haasnoot WH, Ponec M, et al. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res. 1986 Jan;162(1):97-113. PubMed PMID: 2415382.
  2. Weikel W, Wagner R, Moll R. Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in reserve cells. Virchows Arch B Cell Pathol Incl Mol Pathol. 1987;54(2):98-110. PubMed PMID: 2447698.
  3. Smedts F, Ramaekers F, Robben H, Pruszczynski M, van Muijen G, Lane B, et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am J Pathol. 1990 Mar;136(3):657-68. PubMed PMID: 1690513. (Free PMC Article available)
  4. van Niekerk CC, Boerman OC, Ramaekers FC, Poels LG. Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas. Am J Pathol. 1991 Feb;138(2):455-63. PubMed PMID: 1992770. (Free PMC Article available)
  5. Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Link M, Lane B, et al. Keratin expression in cervical cancer. Am J Pathol. 1992 Aug;141(2):497-511. PubMed PMID: 1379783. (Free PMC Article available)
  6. Bauwens LJ, De Groot JC, Ramaekers FC, Veldman JE, Huizing EH. Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol. 1992 Jun;101(6):479-86. PubMed PMID: 1376975.
  7. Van Niekerk CC, Ramaekers FC, Hanselaar AG, Aldeweireldt J, Poels LG. Changes in expression of differentiation markers between normal ovarian cells and derived tumors. Am J Pathol. 1993 Jan;142(1):157-77. PubMed PMID: 7678716. (Free PMC Article available)
  8. van Dorst EB, van Muijen GN, Litvinov SV, Fleuren GJ. The limited difference between keratin patterns of squamous cell carcinomas and adenocarcinomas is explicable by both cell lineage and state of differentiation of tumour cells. J Clin Pathol. 1998 Sep;51(9):679-84. PubMed PMID: 9930073. (Free PMC Article available)
  9. Tabuchi Y, Wada S, Ikegame M, Kariya A, Furusawa Y, Hoshi N, et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim. 2014;63(1):31-44. PubMed PMID: 24521861.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Rat and Zebrafish
Specificity
Specificity:
1C7 reacts exclusively with Cytokeratin 13 which is present in non-cornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia.
Gene ID 3860
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 13 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 2)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 1)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Positive controls for Cytokeratin 13 (1 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 Lysate

Western Blot: Cytokeratin 13 Lysate [NBL1-12380] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT13
  Novus Biologicals Inc.
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