BM5502S Cytokeratin 13 antibody

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1 ml / €260.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Cytokeratin 13

BM5502S

Product Description for Cytokeratin 13

Mouse anti Human Cytokeratin 13.
Presentation: Supernatant
Product is tested for Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections, Frozen Sections.

Properties for Cytokeratin 13

Product Category Primary Antibodies
Target Category
Quantity 1 ml
Synonyms CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13
Presentation Supernatant
Reactivity Hu
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13646
Immunogen:
Cytokeratin purified from Human esophagus
GeneID:
3860
Application Immunoblotting.
Immunofluorescence.
Immunohistochemistry on Frozen Sections: 1/5-1/25 (±1/20), preferable in PBS.
Immunohistochemistry on Paraffin Sections: 1/10 after TUF treatment.
Background Cytokeratin 13 is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus.
Concentration ~20 μg antibody/ml
General Readings
  1. Broers JL, Ramaekers FC, Rot MK, Oostendorp T, Huysmans A, van Muijen GN, et al. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res. 1988 Jun 1;48(11):3221-9. PubMed PMID: 2452687.
  2. van Niekerk CC, Jap PH, Thomas CM, Smeets DF, Ramaekers FC, Poels LG. Marker profile of mesothelial cells versus ovarian carcinoma cells. Int J Cancer. 1989 Jun 15;43(6):1065-71. PubMed PMID: 2659540.
  3. Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55. PubMed PMID: 1690512. (Free PMC Article available)
  4. van Niekerk CC, Jap PH, Ramaekers FC, van de Molengraft F, Poels LG. Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues. J Pathol. 1991 Oct;165(2):145-52. PubMed PMID: 1720817.
  5. van Niekerk CC, Boerman OC, Ramaekers FC, Poels LG. Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas. Am J Pathol. 1991 Feb;138(2):455-63. PubMed PMID: 1992770. (Free PMC Article available)
  6. Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Link M, Lane B, et al. Keratin expression in cervical cancer. Am J Pathol. 1992 Aug;141(2):497-511. PubMed PMID: 1379783. (Free PMC Article available)
  7. van Muijen GN, Ruiter DJ, Franke WW, Achtstätter T, Haasnoot WH, Ponec M, et al. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res. 1986 Jan;162(1):97-113. PubMed PMID: 2415382.
  8. Kopan R, Traska G, Fuchs E. Retinoids as important regulators of terminal differentiation: examining keratin expression in individual epidermal cells at various stages of keratinization. J Cell Biol. 1987 Jul;105(1):427-40. PubMed PMID: 2440897. (Free PMC Article available)
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Preservatives:
10mM Sodium Azide, FCS
State:
Liquid Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human.
Specificity
Specificity:
This Monoclonal Antibody Clone 2D7 does stain non-cornifying squamous epithelium.
In Immunoblotting experiments the antibody recognizes only Keratin 13 (54kD) and does not show any cross reactivity with other keratins.  
The antibody is a useful Marker for histopathology.
Frozen sections give excellent results in immunoperoxidase and immunofluorescence tests.
Cross reactivity:
Vimentin : not detectable
Desmin : not detectable
GFAP : not detectable
Neurofilament : not detectable.
Gene ID 3860

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 13 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 2)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 1)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Positive controls for Cytokeratin 13 (1 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 Lysate

Western Blot: Cytokeratin 13 Lysate [NBL1-12380] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT13
  Novus Biologicals Inc.
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