DM056P Cytokeratin 13 (+ KRT10) antibody

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0.1 mg / €340.00
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Mouse anti Canine, Feline, Human, Zebrafish Cytokeratin 13 DE-K13

Product Description for Cytokeratin 13

Mouse anti Canine, Feline, Human, Zebrafish Cytokeratin 13 DE-K13.
Properties: (+ KRT10)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Flow Cytometry, Paraffin Sections.

Properties for Cytokeratin 13

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms CK-13, CK13, K13, KRT-13, KRT13, Keratin type I cytoskeletal 13, Keratin-13
Presentation Purified
Reactivity Can, Fe, Hu, Ze
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone DE-K13
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P13646
Immunogen:
Cytoskeletal preparation extracted from Human ectocervical epithelium.
GeneID:
3860
Property + KRT10
Isotype control AM03096PU-N
Application Immunoblotting
Immunocytochemistry.
Immunofluorescence.
Immunohistochemistry on Frozen Sections and Paraffin-Embedded tissues.
Flow Cytometry.
Recommended Dilutions: 1/100-1/200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1/100-1/1000 for Immunoblotting applications.
For staining on Paraffin-embedded tissues pretreatment with 0,1% pepsin in 0.1N HCl for 30 min at room temperature is required.
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20.
The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings
  1. Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am J Vet Res. 1992 Mar;53(3):304-14. PubMed PMID: 1375818.
  2. Ivanyi D, Minke JM, Hageman C, Groeneveld E, van Doornewaard G, Misdorp W. Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am J Vet Res. 1993 Jul;54(7):1095-102. PubMed PMID: 7690208.
  3. van Bommel PF, Kenemans P, Helmerhorst TJ, Gallee MP, Ivanyi D. Expression of cytokeratin 10, 13, and involucrin as prognostic factors in low stage squamous cell carcinoma of the uterine cervix. Cancer. 1994 Oct 15;74(8):2314-20. PubMed PMID: 7522949.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Human, Feline, Zebrafish, Canine.
Specificity
Specificity:
DE-K13 reacts with Cytokeratin 10 and 13 in fixed cells and on frozen tissues, but exclusively with cytokeratin 13 in Formalin-Fixed, Paraffin Embedded tissues. 
Cytokeratin 10 is present in keratinizing stratified epithelia and in differentiated areas of highly differentiated squamous cell carcinomas, while Cytokeratin 13 is present in noncornified squamous epithelia, except cornea, and transitional epithelial regions, with the exception of basal cell layers of some stratified epithelia, as well as carcinomas derived from these tissues.
Gene ID 3860
FocusOn and Reviews
Reviews:

Accessory Products

Proteins and/or Positive Controls

Proteins for Cytokeratin 13 (2 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 2)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Cytokeratin 13 (full length, N-term HIS tag, transcript variant 1)

Cytokeratin 13 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Positive controls for Cytokeratin 13 (1 products)

Catalog No. Species Pres. Purity   Source  

Cytokeratin 13 Lysate

Western Blot: Cytokeratin 13 Lysate [NBL1-12380] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for KRT13
  Novus Biologicals Inc.
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