AM50198PU-S Cytokeratin 4+5+6+8+10+13+18 antibody

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0.1 mg / €320.00
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Mouse anti Bovine, Frog, Guinea Pig, Goat, Human, Marmoset, Mouse, Porcine, Rat Cytokeratin 4+5+6+8+10+13+18 SPM583

Product Description for Cytokeratin 4+5+6+8+10+13+18

Mouse anti Bovine, Frog, Guinea Pig, Goat, Human, Marmoset, Mouse, Porcine, Rat Cytokeratin 4+5+6+8+10+13+18 SPM583.
Presentation: Purified
Product is tested for Frozen Sections, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Paraffin Sections, Western blot / Immunoblot.

Properties for Cytokeratin 4+5+6+8+10+13+18

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Cytokeratin pan-reactive, Keratin type I cytoskeletal 1, Keratin type I cytoskeletal 10, Keratin type I cytoskeletal 18, Keratin type II cytoskeletal 4, Keratin type II cytoskeletal 5, Keratin type II cytoskeletal 6, Keratin type II cytoskeletal 8, pan Keratin
Presentation Purified
Reactivity Bov, Frog, GP, Gt, Hu, Marmoset, Ms, Por, Rt
Applications C, F, ICC/IF, P, WB
Clonality Monoclonal
Clone SPM583
Host Mouse
Isotype IgG1
Molecular weight Multiple
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Keratin-enriched preparation from cultured Human A431.
Application Flow Cytometry: 0.5-1 µg/million cells.
Immunofluorescence: 0.5-1 µg/ml. 
Western Blotting: 0.5-1 µg/ml.
Immunohistochemistry on Frozen and Formalin-Fixed Paraffin Sections: 0.5-1 µ/ml for 30 minutes at RT. 
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Positive Control: A431 cells, Skin, Colon carcinoma.
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Concentration 0.2 mg/ml
General Readings
  1. Bártek J, Vojtĕsek B, Stasková Z, Bártková J, Kerekés Z, Rejthar A, et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24. PubMed PMID: 1716305.
  2. Lane EB, Alexander CM. Use of keratin antibodies in tumor diagnosis. Semin Cancer Biol. 1990 Jun;1(3):165-79. PubMed PMID: 1715788.
  3. Bártková J, Bártek J, Lukás Z, Vojtĕsek B, Stasková Z, Bursová H, et al. Effects of tissue fixation conditions and protease pretreatment on immunohistochemical performance of a large series of new anti-keratin monoclonal antibodies: value in oncopathology. Neoplasma. 1991;38(4):439-46. PubMed PMID: 1717857.
  4. Kasper M. Heterogeneity in the immunolocalization of cytokeratin specific monoclonal antibodies in the rat eye: evaluation of unusual epithelial tissue entities. Histochemistry. 1991;95(6):613-20. PubMed PMID: 1713203.
Storage Store undiluted at 2-8°C.
Shelf life: one year from despatch.
Protein A/G Chromatography
Buffer System:
10mM PBS
0.05% Sodium Azide
0.05% BSA
Liquid purified IgG fraction from Bioreactor Concentrate
This Monoclonal Antibody recognizes Cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Cellular Localization: Cytoplasmic.
Human, Cow, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset and Pig.

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