NB100-78482 Cytokeratin 7+17 antibody

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Mouse anti Human, Porcine Cytokeratin 7+17 C-46

Product Description for Cytokeratin 7+17

Mouse anti Human, Porcine Cytokeratin 7+17 C-46.
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin 7+17

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms Keratin, type I cytoskeletal 17, type II cytoskeletal 7
Presentation Aff - Purified
Reactivity Hu, Por
Applications ICC/IF, IP, P, WB
Clonality Monoclonal
Clone C-46
Host Mouse
Isotype IgG1
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Cytoskeleton preparation derived from Hela cells
Application Immunocytochemistry, Immunohistochemistry-Paraffin, immunoprecipitation, Western Blot, Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 mug antibody per 5 ml antibody dilution buffer for each mini-gel. Immunohistochemistry-paraffin. It is recommended that the reagent be titrated for optimal performance for each application., immunoprecipitation , Western Blot , Immunocytochemistry , Immunohistochemistry-Paraffin
Background Cytokeratins are proteins that are involved in the cytoskeleton. The cytokeratin proteins are heterotetramers composed of two type I and two type II subunits. Cytokeratin 17 is a type I protein and cytokeratin 7 is a type II intermediate filament protein, each protein contains three coiled-coil domains. Cytokeratin 7 has an approximate molecular weight of 54 kD; cytokeratin 17 has an approximate molecular weight of 46 kD. Cytokeratin 17 is expressed in the basal cells of complex epithelia, but not in stratified or simple epithelia. Cytokeratin 7 is expressed in the epidermis, bronchus, and mesothelium but not in the colon, exocervix or liver. Mutations in cytokeratin 17 have been shown to result in pachyonychia congenital. Cytokeratin 17 has been shown to interact with the EGF receptor. Cytokeratin 7 has been shown to interact with the EGF receptor and heat shock protein 70 kD1A. The C-46 monoclonal antibody recognizes human, bovine and porcine (but not mouse, rat, or rabbit) cytokeratin 7 and cytokeratin 17 and is useful for Western blotting.This antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), and immunocytochemistry.
General Readings
  1. Glass, C., et al., 1988. J. Cell. Biol. 107:1337.2. Troyanovsky, S. M., et al., 1992. Eur. J. Cell Biol. 59:127.3. Terrinoni, A., et al., 2001. J. Invest. Dermatol. 117:1391.
Storage Store at 4C. Do not freeze.
Immunogen affinity purified
Buffer System:
0.09% Sodium Azide
Immunogen affinity purified
Aff - Purified
Cytokeratin 7/17 (C-46)
Human, Porcine

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