BM6001P Cytokeratin (basal cell) antibody

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0.1 mg / €320.00
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Mouse anti Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Quail, Rabbit, Rat, Zebrafish Cytokeratin (basal cell) RCK103

BM6001P

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  • BM6001P

Product Description for Cytokeratin (basal cell)

Mouse anti Canine, Chicken, Guinea Pig, Hamster, Human, Porcine, Quail, Rabbit, Rat, Zebrafish Cytokeratin (basal cell) RCK103.
Presentation: Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Frozen Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for Cytokeratin (basal cell)

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Can, Chk, GP, Hst, Hu, Por, Quail, Rb, Rt, Ze
Applications C, F, ICC/IF, WB
Clonality Monoclonal
Clone RCK103
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
A mix of cell preparations containing Human cytokeratins.
Isotype control AM03095PU-N, SM10P (for use in human samples), SM20P (for use in rat samples)
Application Western blot: 1/100-1/1000. 
Immunohistochemistry on Frozen tissues: 1/100-1/200 with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. 
Immunocytochemistry. 
Immunofluorescence.
 
Flow Cytometry (1/100-1/200).
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium.
Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Concentration 1.0 mg/ml
General Readings 1. Feitz, W. F., Debruyne, F. M., Vooijs, G. P., Herman,C. J., and Ramaekers, F. C. (1986). Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues, J Urol 136, 922-31.
2. Verhagen, A. P., Aalders, T. W., Ramaekers, F.C.,Debruyne, F. M., Schalken, J. A. (1988). Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration, Prostate 13, 25-38.
3. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G.N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract, Histochemistry 91, 151-9.
4. Smedts, F., Ramaekers, F., Robben, H.,Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I.,and Vooijs, P. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68.
5. Kuijpers, W., Tonnaer, E. L., Peters, T. A., and Ramaekers, F. C. (1991).
Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig, Hear Res 52, 133-46.
6. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-) endocrine tissues, choroids plexus and spinal cord, J Vet Med 39, 721-40.
7. Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61.
8. Verhagen, A. P., Ramaekers, F. C., Aalders, T. W., Schaafsma, H. E., Debruyne, F. M., and Schalken, J. A. (1992). Colocalization of basal and luminal celltype cytokeratins in human prostate cancer, Cancer Res 52, 6182-7.
9. van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D., and Schalken, J. (2000). Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy, Lab Invest 80, 1251-8.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freeze-thaw cycles.
Shelf life: One year from despatch.
Format
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
State:
Liquid purified IgG fraction
Purified
Species Reactivity
Species reactivity (tested):
Canine, Chicken, Guinea Pig, Hamster, Human, Quail, Rabbit, Rat, Swine, Zebrafish.
Specificity
Specificity:
Clone RCK103 is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5.
This monoclonal antibody stains basal cells in combined and stratified epithelial tissues.
It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.
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