AM01378PU-N Cytokeratin type I (acidic) antibody

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Mouse anti Human Cytokeratin type I (acidic) AE1


Product Description for Cytokeratin type I (acidic)

Mouse anti Human Cytokeratin type I (acidic) AE1.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytokeratin type I (acidic)

Product Category Primary Antibodies
Quantity 0.2 ml
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone AE1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Human epidermal keratins
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry.
Western blot (1:1000 - 1:3000).
Background Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. The basic keratins have molecular weights of 65 - 67, 64, 59, 58, 56 and 52kDa. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Protocols Western Blotting Protocol

1. Transfer gel to PDVF or nitrocellulose membrane
2. Place membrane in plastic tray in blocking buffer for one hour with agitation
3. Rinse in wash buffer
4. Incubate in wash buffer plus primary antibody for one hour
5. Wash 6 X 5 minutes with wash buffer
6. Incubate in wash buffer plus secondary antibody for one hour
7. Wash 6X 5 minutes with wash buffer
8. Detect (e.g. ECL, Amersham according to manufacturers instructions)

Wash buffer:
PBS + 0.1% Tween 20

Blocking buffer:
Wash buffer + 5% dried milk powder
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein G chromatography
Buffer System:
PBS containing 0.09% sodium azide
Liquid purified Ig fraction
The antibody recognizes most human acidic (type I) keratins K10, K14, K15, K16, K19.
Other species not tested.

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