AM32083PU-N Cytomegalomavirus (CMV) - LA antibody

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Mouse anti Human Cytomegalomavirus (CMV) - LA BM219


Product Description for Cytomegalomavirus (CMV) - LA

Mouse anti Human Cytomegalomavirus (CMV) - LA BM219.
Presentation: Purified
Product is tested for Paraffin Sections, Immunocytochemistry/Immunofluorescence, Enzyme Immunoassay, Frozen Sections, Western blot / Immunoblot.

Properties for Cytomegalomavirus (CMV) - LA

Product Category Primary Antibodies
Quantity 1 ml
Presentation Purified
Reactivity Hu
Applications C, E, ICC/IF, P, WB
Clonality Monoclonal
Clone BM219
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Isotype control AM03095PU-N, SM10P (for use in human samples)

ELISA: 1/500-1/1000. 
Western Blot. 
Immunofluorescence (1/50).  This antibody is useful for the detection of the late stage of cytomegalovirus infection.
Immunohistochemistry on Fresh Cells and Paraffin Embedded Tissue Sections (1/50). If a too low signal is obtained, it is advised to use as second antibody a biotinylated F(ab')2 preparation of anti-Mouse IgG followed by a layer with streptavidin-Biotin-HRP complex and silver enhancement.


Cytomegalovirus (CMV) is a member of the Herpes virus family. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid which is embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope that is decorated with virus-specific glycoprotein spikes. The viral genes are co-ordinately expressed in groups at various times after infection. Early viral proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection prior to the commencement of viral DNA replication. This is followed by expression of the early intermediate genes, which encode enzymes required for viral DNA replication. After 48 to 72 hours, a number of late viral antigens may be demonstrated in the nuclei and cytoplasm of infected cells. CMV strain AD169 is a laboratory-adapted strain. It appears to lack a 15kb region of the 200kb genome that is present in clinical isolates. This region contains 19 open reading frames whose functions have yet to be elucidated. AD169 is also unique in that it is unable to enter latency and nearly always assumes lytic growth upon infection.

Concentration 0.1 mg/ml
General Readings
  1. Bruning JH, Debie WH, Dormans PH, Meijer H, Bruggeman CA. The development and characterization of monoclonal antibodies against rat cytomegalovirus induced antigens. Arch Virol. 1987;94(1-2):55-70. PubMed PMID: 2437886.
Storage Store the antibody undiluted  at 2-8°C.
Shelf life: one year from despatch.
Buffer System:
0.09% Sodium Azide
0.1% BSA
Liquid purified IgG fraction
Reacts with Cytomegalovirus.
The specificity of the antibody was ascertained by Immunofluorescence on the AD 169 strain (ATCC VR-538), field isolates and a large number of wild strain, immediate early, early and late antigen preparations.
The antibody recognizes in Western blot a structural antigen of 21-23 kD.
This antigen can be detected by Fluorescence assay at 96 hours after infection. The antibody gives a specific cytoplasmic perinuclear fluorescence only in case of late antigen (LA) expression. It does not crossreact with other Herpes viruses.

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