BM3106 Cytomegalovirus (CMV) - LA antibody

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Mouse anti Cytomegalovirus (CMV) - LA BM219


Product Description for Cytomegalovirus (CMV) - LA

Mouse anti Cytomegalovirus (CMV) - LA BM219.
Presentation: Azide Free
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Cytomegalovirus (CMV) - LA

Product Category Primary Antibodies
Quantity 1 ml
Presentation Azide Free
Applications C, ICC/IF, P, WB
Clonality Monoclonal
Clone BM219
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Application Suitable for use in Immunohistochemistry (1:50, on fresh cells and on paraffin embedded tissue sections), Western blots and ELISA (1:500-1:1000).
Can be used for standard Immunofluorescence and Immunohistochemical procedures.
In order to prevent aspecific staining, the second antibody either labeled with fluorochrome or with an enzyme should be a F(ab')2 preparation of anti-mouse IgG (preferentially anti-Fc).
In case a low signal is obtained in immunohistochemistry, it is advised to use as second antibody a biotinylated F(ab')2 preparation of anti-mouse IgG followed by a layer with streptavidin-Biotin-HRP complex and silver enhancement.
Background Cytomegalovirus is a member of the herpes virus group, which includes herpes simplex virus types 1 and 2, varicella zoster virus (which causes chicken pox), and Epstein Barr virus (which causes infectious mononucleosis). These viruses share a characteristic ability to remain dormant within the body over a long period.
Concentration 100 µg/ml (OD280 nm, E0.1% = 1.4)
General Readings Bruning J.H., et. al., (1987), Arch. Viro., 94, 55.
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Buffer System:
PBS containing 0.1% BSA and 0.09% sodium azide as preservative.
Liquid purified IgG fraction.
Azide Free
Specificity was ascertained by Immunofluorescence on the AD169 strain (ATCC,VR-538), field isolates and a large number of wild strain, immediate early, early and late antigen preparations.
Recognizes in Western blot a structural antigen of 21-23 kD (can be detected by fluorescence assay at 96 hours after infection).
Gives a specific cytoplasmic perinuclear fluorescence only in case of late antigen (LA) expression.
No cross-reactivity with other herpes viruses.

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