discontinued

BM2210 Cytotoxic Effector T Cells (NK cells) antibody

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0.2 mg / €530.00

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Mouse anti Human Cytotoxic Effector T Cells (NK cells) C1.7

BM2210

Product Description for Cytotoxic Effector T Cells (NK cells)

Mouse anti Human Cytotoxic Effector T Cells (NK cells) C1.7.
Presentation: Purified
Product is tested for Functional assay, Flow Cytometry.

Properties for Cytotoxic Effector T Cells (NK cells)

Product Category Primary Antibodies
Quantity 0.2 mg
Presentation Purified
Reactivity Hu
Applications F, FN
Clonality Monoclonal
Clone C1.7
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Cultured human NK-cells.
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow Cytometry or Fluorescent Microscopy: 2 µg antibody 5x10e5 cells/test or 100 µl whole blood
This antibody can be used as NK marker in the CD3 population and as marker of cytotoxic effector Cells in the CD3+ CD8+ population.
Cell-mediated cytotoxicity Assays: In antibody-redirected lysis assays, FcgammaR P815x2 Cells where used as targets and tests where performed in microtiter plates (10e4 cells/well). 0.1 µg/ml of mAb C1.7 was used for induced NK cell-mediated cytotoxicity.
Concentration 0.2 mg/ml (prior to lyophilization)
General Readings
  1. Valiante NM, Trinchieri G. Identification of a novel signal transduction surface molecule on human cytotoxic lymphocytes. J Exp Med. 1993 Oct 1;178(4):1397-406. PubMed PMID: 8376943. (Free PMC Article available)
Storage Store lyophilized product at 2-8°C.
After reconstitution, aliquot and store at -20°C.
Avoid multiple freeze/thaw cycles.
Addition of 0.09% (w/v) sodium azide is recommended for storage of the reconstituted form.
Shelf life: one year from despatch.
Format
Buffer System:
PBS with 1 mg/ml BSA as stabilizer and without preservatives.
State:
Lyophilized purified Ig fraction.
Purified
Reconstitution:
Restore with 1 ml of distilled water.
Specificity
Specificity:
The C1.7 monoclonal antibody recognizes a 38 kDa novel cell surface signal transduction molecule (p38) found on all human NK-cells.
It induces a granule independent killing mechanism by NK effector cells. It is also found on gamma 8 TCR T-cells and approximately half of peripheral alpha/beta TCR CD8+ T-cells. It is not found on B-, CD4+ T-, or naïve CD8+ T-cells. Upon activation, most CD8+ T-cells become C1.7 positive. However, a small but significant minority of CD8+ T-cells remain C1.7 negative. This subset is associated with lower cytotoxic activity, lower gamma IFN production and high IL4/Il10 production. Therefore, this mAb may be useful in defining subsets of CD8+ T-cells and studying a novel cytotoxic mechanism on NK-cells.

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