4D30-DD255 D-Dimer antibody

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1 mg / €410.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti D-Dimer DD255

4D30-DD255

Product Description for D-Dimer

Mouse anti D-Dimer DD255.
Presentation: Purified
Product is tested for Western blot / Immunoblot, Enzyme Immunoassay.

Properties for D-Dimer

Product Category Primary Antibodies
Quantity 1 mg
Synonyms Fibrin breakdown product, Fibrinogen Degradation Product D
Presentation Purified
Applications E, WB
Clonality Monoclonal
Clone DD255
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer HyTest Ltd.
Manufacturer certificates ISO 9001:2008

Datasheet Extract

Immunogen
Immunogen:
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.
Isotype control AM03095PU-N
Application

Immunoassays for the quantitative determination of D-dimer and high molecular weight fibrin degradation products.

All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 and DD189 interact with beta-chain of D-dimer in Western blotting under reducing conditions. MAbs DD93 and DD255 interact with gamma-chain of D-dimer in Western blotting under reducing conditions.

Recommended pairs to be used in a sandwich immunoassay for D-dimer detection in human plasma (capture-detection):

One-step sandwich immunoassays:

DD189 – DD255 (Equal specificity to D-dimer and high MW fibrin degradation products).
DD2 – DD41 (Slightly more specific to high MW fibrin degradation products at high concentrations).

Two-step sandwich immunoassays*:

DD2 – DD4 (Approximately equal specificity to D-dimer and high MW fibrin degradation products).

(*To be analyzed in a two-step sandwich immunoassay, plasma must be diluted 1:1 or more with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.)

Storage Store at 4°C.
Format
Purification:
Chromatography on protein A Sepharose
Buffer System:
PBS, pH 7.4
Preservatives:
0.1% Sodium Azide
State:
Purified
Specificity
Specificity:

All MAbs recognize D-dimer and high molecular weight fibrin degradation products.

MAb DD93 recognizes a cross-linked region of D-dimer.

MAbs DD1, DD2, DD3, DD22, DD41, DD44, DD46, DD93, DD189 and DD255 do not cross-react with fibrinogen.

MAbs DD4, DD5 and DD6 show cross-reaction with fibrinogen.

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