NB100-1924 DAF-3 antibody

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0.1 ml / €360.00
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Quick Overview

Rabbit anti C. elegans DAF-3

Product Description for DAF-3

Rabbit anti C. elegans DAF-3.
Presentation: Serum
Product is tested for ChIP assay, Paraffin Sections, Immunoprecipitation, Western blot / Immunoblot.

Properties for DAF-3

Product Category Primary Antibodies
Quantity 0.1 ml
Synonyms Abnormal dauer formation protein 3 - isoform c, DAF3, F25E2.5
Presentation Serum
Reactivity Ce
Applications ChIP, IP, P, WB
Clonality Polyclonal
Host Rabbit
Shipping to Not USA/Canada
PDF datasheet View Datasheet
Manufacturer Novus Biologicals Inc.

Datasheet Extract

Immunogen
Swiss Prot Num:
O17532
Immunogen:
A synthetic peptide made to the C-terminus (within residues 700-796) of the C.elegans DAF-3 protein.
GeneID:
180431
Application chromatin immunoprecipitation, immunoprecipitation, Western Blot, This antibody is useful for Western blot. The molecular weight of this protein is ~91 kD. Ch Immunoprecipitation - (See Deplancke, et al. Cell. 2006), chromatin immunoprecipitation , immunoprecipitation , Western Blot 1:1,000-1:5,000
Background Signals from TGF-beta superfamily receptors are transduced to the nucleus by Smad proteins, which transcriptionally activate target genes. In Caenorhabditis elegans (C.elegans), defects in a TGF-beta-related pathway cause a reversible developmental arrest and metabolic shift at the dauer larval stage. DAF-3 transgenes interfere with dauer formation, playing a role in the nucleus. DAF-3 is unique in that it is antagonized, rather than activated, by a TGF-beta pathway.
General Readings
  1. Deplancke, B, et al. Cell. 125(6): 1193-205 (2006) [ChIP]
Storage Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Format
Purification:
Whole antisera
Buffer System:
0.05% Sodium Azide
Purity:
Whole antisera
State:
Serum
Specificity
Specificity:
DAF-3
Species:
C. elegans
Gene ID 180431

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