PP1229B1 Defensin beta 3 antibody

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25 µg / €230.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human Defensin beta 3

PP1229B1

Product Description for Defensin beta 3

Rabbit anti Human Defensin beta 3.
Presentation: Biotin
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Defensin beta 3

Product Category Primary Antibodies
Target Category
Quantity 25 µg
Synonyms BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
Presentation Biotin
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P81534
Immunogen:
E.coli derived recombinant Human Beta Defensin-3 (Cat.-No PA184).
GeneID:
414325
Add. information Molecular weight: ~150 kDa
Application Direct ELISA: To detect Human BD-3 by Direct ELISA (using 100µl/well) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated antibody allows the detection of at least 0.2-0,4 mg/well of recombinant Humand BD-3.
Sandwich ELISA:
To detect Human BD-3 by Sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjugation with Purified Anti-Human BD-3 antibody (Cat.-No PP1229P) as a Capture antibody, allows the detection of at least 0.2-0.4 mg/well of recombinant Humand BD-3.
Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma.
Beta Defensin 3 belongs to the beta-defensin family.
Storage

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Label:
Biotin
Purification:
Affinity Chromatography
Buffer System:
BPS, pH 7.2 without preservatives
State:
Lyophilized (sterile filtered) purified Ig fraction
Reconstitution:
Centrifuge vial prior to opening.
Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml
Specificity
Specificity:
This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
Gene ID 414325

Accessory Products

Proteins and/or Positive Controls

Proteins for Defensin beta 3 (9 products)

Catalog No. Species Pres. Purity   Source  

Defensin beta 3

Defensin beta 3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €139.00
  OriGene Technologies, Inc.

Defensin beta 3

Defensin beta 3 Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Defensin beta 3

Defensin beta 3 Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Defensin beta 3

Defensin beta 3 Human
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human
  Abnova Taiwan Corp.

Defensin beta 3 (full length, oxidized)

Defensin beta 3 Mouse Purified > 95 % Synthetic
  Alpha Diagnostic Intl. Inc.

Defensin beta 3 (full length, linear - no disulfides)

Defensin beta 3 Mouse Purified > 95 % Synthetic
  Alpha Diagnostic Intl. Inc.

Positive controls for Defensin beta 3 (3 products)

Catalog No. Species Pres. Purity   Source  

DEFB103A 293T Cell Transient Overexpression Lysate(Denatured)

DEFB103A 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-DEFB103A antibody (H00055894-B01) by Western Blots.
  Abnova Taiwan Corp.

DEFB103A overexpression lysate

DEFB103A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

DEFB103B overexpression lysate

DEFB103B overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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