PP1229P1 Defensin beta 3 antibody

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50 µg / €240.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human Defensin beta 3

Product Description for Defensin beta 3

Rabbit anti Human Defensin beta 3.
Presentation: Aff - Purified
Product is tested for Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Defensin beta 3

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms BD3, Beta-defensin 103, DEFB103, DEFB103A, DEFB103B, DEFB3, Defensin beta 103, Defensin-like protein, HBD3, HBP3
Presentation Aff - Purified
Reactivity Hu
Applications E, WB
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P81534
Immunogen:
E.coli derived Recombinant Human Beta Defensin-3.
GeneID:
414325
Add. information Molecular weight: ~150 kDa
Application Sandwich ELISA: To detect Human BD-3 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. Antibody PP1229P, in conjunction with biotinylated anti-Human BD-3 (Cat.-No PP1229B), allows the detection of at least 0.2-0.4 ng/well of recombinant Human BD-3.
Western Blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Background Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma.
Beta Defensin 3 belongs to the beta-defensin family.
Product Citations
Purchased from Acris:
  1. Gläser R, Becker K, von Eiff C, Meyer-Hoffert U, Harder J. Decreased susceptibility of Staphylococcus aureus small-colony variants toward human antimicrobial peptides. J Invest Dermatol. 2014 Sep;134(9):2347-50. doi: 10.1038/jid.2014.176. Epub 2014 Apr 9. PubMed PMID: 24717245.
General Readings
  1. Ghosh SK, Gerken TA, Schneider KM, Feng Z, McCormick TS, Weinberg A. Quantification of human beta-defensin-2 and -3 in body fluids: application for studies of innate immunity. Clin Chem. 2007 Apr;53(4):757-65. Epub 2007 Feb 22. PubMed PMID: 17317882.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography employing immobilized Human BD-3 matrix
Buffer System:
PBS, pH 7.2 without preservatives.
State:
Lyophilized (sterile filtered) purified Ig fraction
Aff - Purified
Reconstitution:
Centrifuge vial prior to opening.
Restore with sterile water to a concentration of 0.1-1.0 mg/ml.
Specificity
Specificity:
This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
Gene ID 414325

Accessory Products

Proteins and/or Positive Controls

Proteins for Defensin beta 3 (9 products)

Catalog No. Species Pres. Purity   Source  

Defensin beta 3

Defensin beta 3 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
20 µg / €139.00
  OriGene Technologies, Inc.

Defensin beta 3

Defensin beta 3 Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Defensin beta 3

Defensin beta 3 Human Purified > 98 % pure by SDS-PAGE and HPLC analyses E. coli
  Acris Antibodies GmbH

Defensin beta 3

Defensin beta 3 Human
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Defensin beta 3

Defensin beta 3 Human
  Abnova Taiwan Corp.

Defensin beta 3 (full length, oxidized)

Defensin beta 3 Mouse Purified > 95 % Synthetic
  Alpha Diagnostic Intl. Inc.

Defensin beta 3 (full length, linear - no disulfides)

Defensin beta 3 Mouse Purified > 95 % Synthetic
  Alpha Diagnostic Intl. Inc.

Positive controls for Defensin beta 3 (3 products)

Catalog No. Species Pres. Purity   Source  

DEFB103A 293T Cell Transient Overexpression Lysate(Denatured)

DEFB103A 293T Cell Transient Overexpression Lysate(Denatured) Transient overexpression cell lysate was tested with Anti-DEFB103A antibody (H00055894-B01) by Western Blots.
  Abnova Taiwan Corp.

DEFB103A overexpression lysate

DEFB103A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

DEFB103B overexpression lysate

DEFB103B overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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