AP51240PU-N DENND1B (N-term) antibody

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0.4 ml / €370.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Rabbit anti Human DENND1B

AP51240PU-N

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  • AP51240PU-N
  • AP51240PU-N

Product Description for DENND1B

Rabbit anti Human DENND1B.
Properties: (N-term)
Presentation: Aff - Purified
Product is tested for Flow Cytometry, Paraffin Sections, Western blot / Immunoblot.

Properties for DENND1B

Product Category Primary Antibodies
Target Category
Quantity 0.4 ml
Synonyms C1orf218, Connecdenn 2, DENN domain-containing protein 1B, FAM31B
Presentation Aff - Purified
Reactivity Hu
Applications F, P, WB
Clonality Polyclonal
Host Rabbit
Isotype Ig
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6P3S1
Immunogen:
KLH conjugated synthetic peptide between 46-76 amino acids from the N-terminal region of Human DENND1B.
GeneID:
163486
Property N-term
Add. information Molecular Weight: 48260 Da
Application

ELISA: 1/1000.
Western Blot: 1/100-1/500. 
Immunohistochemistry on Paraffin Sections: 1/50-1/100.

Background
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).
Concentration lot specific
General Readings
  1.  Marat, A.L., et al. J. Biol. Chem. 285(14):10627-10637 (2010). 
  2. Sleiman, P.M., et al. N. Engl. J. Med. 362(1):36-44 (2010)
  3. Venter, J.C., et al. Science 291(5507):1304-1351 (2001).
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Format
Purification:
Protein A column, followed by peptide affinity purification
Buffer System:
PBS
Preservatives:
0.09% (W/V) Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody recognizes Human DENND1B (N-term).
Gene ID 163486

Accessory Products

Proteins and/or Positive Controls

Proteins for DENND1B (3 products)

Catalog No. Species Pres. Purity   Source  

DENND1B (full length, N-term HIS tag, transcript variant 2)

DENND1B Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

DENND1B

DENND1B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

DENND1B

DENND1B Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for DENND1B (3 products)

Catalog No. Species Pres. Purity   Source  

DENND1B 293T Cell Transient Overexpression Lysate(Denatured)

DENND1B 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

DENND1B Lysate

Western Blot: DENND1B Lysate [NBL1-08314] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for C1orf218 Protein
  Novus Biologicals Inc.

DENND1B overexpression lysate

DENND1B overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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