DM3539P Desert hedgehog / DHH (N-term) antibody

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0.1 mg / €440.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Rat anti Mouse Desert hedgehog / DHH 6A12

DM3539P

Product Description for Desert hedgehog / DHH

Rat anti Mouse Desert hedgehog / DHH 6A12.
Properties: (N-term)
Presentation: Purified
Product is tested for Western blot / Immunoblot.

Properties for Desert hedgehog / DHH

Product Category Primary Antibodies
Target Category
Quantity 0.1 mg
Synonyms HHG-3
Presentation Purified
Reactivity Ms
Applications WB
Clonality Monoclonal
Clone 6A12
Host Rat
Isotype IgG2
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q61488
Immunogen:
Purified Mouse Recombinant DHH N-terminal fragment
GeneID:
13363
Property N-term
Application Western Blot: 1/500 - 1/1000.
Background Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa Nterminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling. Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis. It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules. In humans, mutations of Dhh are associated with pure gonadal dysgenesis. Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum. Its upregulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis. Dhh is also expressed by Schwann cells and is upregulated following nerve injury. It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month 
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Protein A/G affinity chromatography
Buffer System:
0.2 μm filtered PBS solution
Stabilizers:
None
State:
Lyophilized purified IgG fraction from culture supernatant
Purified
Reconstitution:
Restore with 200µl sterile PBS and the final concentration is 500µg/ml.
Specificity
Specificity:
This antibody detects Mouse DHH N-terminal polypeptide, but not C-terminal one in Western blotting.
Species:
Mouse.
Gene ID 13363

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