discontinued

DP3113 Desmin antibody

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0.5 ml / €120.00

Quick Overview

Rabbit anti Human, Porcine Desmin

DP3113

Product Description for Desmin

Rabbit anti Human, Porcine Desmin.
Presentation: Ig Fraction
Product is tested for Frozen Sections, Paraffin Sections.

Properties for Desmin

Product Category Primary Antibodies
Quantity 0.5 ml
Presentation Ig Fraction
Reactivity Hu, Por
Applications C, P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Biomeda

Datasheet Extract

Immunogen
Immunogen:
Human desmin.

Format: This antibody is supplied as Rabbit serum containing 20 mM tris-borate, 150 mM Sodium Chloride, BMC-6 carrier polysaccharides, carrier protein, and 0.05% Sodium Azide, pH 7.5.
Application Immunohistochemistry: 1:75-1:150. This antibody can be used on frozen tissue sections or formalin-fixed paraffin embedded tissue sections (requires pretreatment with trypsin or high temperature antigen unmasking using 10 mM Citrate buffer pH 6.0 for 10-20 min followd by cooling to RT for 10-20 mins; Prolonged fixation in buffered formalin can destroy the Epitope; Recommended positive control: Heart Muscle, Smooth and Skeletal Muscle, Leiomyoma and Rhabdomyomas). Other applications not tested. Optimal dilutions of this antibody are dependent on conditions and should be determined by the user.
Concentration 3 mg/ml
General Readings
  1. Geisler N, Weber K. Purification of smooth-muscle desmin and a protein-chemical comparison of desmins from chicken gizzard and hog stomach. Eur J Biochem. 1980 Oct;111(2):425-33. PubMed PMID: 7460905.
  2. Lazarides, E., Ann. Rev. Biochem., 80: 166-182, 1982
  3. Bignamis, E., et al, J. Histochem. an
Storage Store the antibody at 4°C. Do not freeze! Shelf life: one year from despatch.

Aliquoting Instructions: Do not dilute the entire reconstituted solution at once. Withdraw aliquots as needed with a micropipette and keep concentrated stock at 4°C. Dilute according to the particular application being used. In general, the 0.05M borate pH 8.0 containing 0.15M sodium chloride, 0.02% sodium azide, is a good dilutent to use with most antibodies. Avoid diluting the entire contents of the vial at once since the diluted solution may have reduced stability.
Format
State:
Ig Fraction
Specificity
Specificity:
Desmin is the main intermediate filament in mature skeletal, cardiac, and smooth muscle cells. In immunoblots, this antibody reacts with a 53 kD polypeptide. This antibody cross-reacts with most mammalian desmins. It does not react with vimentin or human cytokeratins.
Reacts with Human, Porcine. Others not tested.

Mol. Wt. of Antigen: 53kD

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