BM5102 Desmocollin-1 antibody

See related secondary antibodies

Search for all "Desmocollin-1"

50 µg / €330.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Human Desmocollin-1 Dsc-1-U100

BM5102

Product Description for Desmocollin-1

Mouse anti Human Desmocollin-1 Dsc-1-U100.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Desmocollin-1

Product Category Primary Antibodies
Target Category
Quantity 50 µg
Synonyms CDHF1, Cadherin family member 1, DG2/DG3, DSC1, Desmosomal glycoprotein 2/3
Presentation Purified
Reactivity Hu
Applications C, P, WB
Clonality Monoclonal
Clone Dsc-1-U100
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q08554
Immunogen:
Synthetic peptide corresponding to sequence present within the intracellular part of Human Desmocollin 1
AA Sequence:
HQTLESVKGVGQGDTGRYATDWQC
GeneID:
1823
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Imunoblotting (Western).
Immunohistochemistry on Frozen Sections:
For better resolution on Frozen Sections preincubation (directely after fixation) with 0.05-0.2% Triton X-100, for 5-10 min, depending on tissue type is recommended (see Ref.2). Without detergent pretreatment a cytoplasmic component might cross-react (e.g. in fibroblasts).
Immunohistochemistry on Paraffin-Embedded Sections (after microwave treatment):
A slight crossreactivity with a lymphocyte subpopulation is abolished by mild trypsinization after the microwave step.
Working Dilution: After reconstitution dilute further 1/10 in PBS (pH 7.4) for Immunohistochemistry.
Incubation Time: 1h at RT for Immunohistochemistry or overnight at 2-8°C.
Background Desmocollin 1 is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It may contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. Desmocollin 1 is linked to the keratinization of epithelial tissues.
General Readings
  1. Nuber UA, Schäfer S, Stehr S, Rackwitz HR, Franke WW. Patterns of desmocollin synthesis in human epithelia: immunolocalization of desmocollins 1 and 3 in special epithelia and in cultured cells. Eur J Cell Biol. 1996 Sep;71(1):1-13. PubMed PMID: 8884173.
  2. Schäfer S, Stumpp S, Franke WW. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 1996 May;60(2):99-108. PubMed PMID: 8641550.
  3. Moll I, Houdek P, Schäfer S, Nuber U, Moll R. Diversity of desmosomal proteins in regenerating epidermis: immunohistochemical study using a human skin organ culture model. Arch Dermatol Res. 1999 Jul-Aug;291(7-8):437-46. PubMed PMID: 10482015.
  4. Kurzen H, Moll I, Moll R, Schäfer S, Simics E, Amagai M, et al. Compositionally different desmosomes in the various compartments of the human hair follicle. Differentiation. 1998 Sep;63(5):295-304. PubMed PMID: 9810708.
  5. Akat K, Mennel HD, Kremer P, Gassler N, Bleck CK, Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 2003 Oct;106(4):337-47. Epub 2003 Jul 5. PubMed PMID: 12845453.
Storage Stable for one year after reconstitution when stored at 2-8°C.
For extended storage keep in aliquots at -20°C.
Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS buffer, pH 7.4 containing 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in 1 ml dist. water.
Specificity
Specificity:
Reacts with 111 kD (form "a") and 103 kD (form "b") polypeptides (Desmocollin 1 splice forms, both at pI 5.2) from Human epidermal desmosomes.
Mab localizes Dsc1 present in
- suprabasal layers of interfollicular epidermis
- specific cell layers (e.g. in hair follicle root sheath)
- Hassall bodies (spheroidal formations of densely packed reticulum-derived cells in the thymus).
Species:
Human.
Gene ID 1823

Accessory Products

Proteins and/or Positive Controls

Positive controls for Desmocollin-1 (1 products)

Catalog No. Species Pres. Purity   Source  

DSC1 overexpression lysate

DSC1 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.
  • LinkedIn