AM32585PU-N Disialoganglioside GD3 antibody

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Mouse anti Human Disialoganglioside GD3 MB3.6


Product Description for Disialoganglioside GD3

Mouse anti Human Disialoganglioside GD3 MB3.6.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for Disialoganglioside GD3

Product Category Primary Antibodies
Quantity 0.1 mg
Presentation Purified
Reactivity Hu
Applications C, F, ICC/IF, P
Clonality Monoclonal
Clone MB3.6
Host Mouse
Isotype IgG3
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

FM9 melanoma cells.
Isotype control AM03097PU-N
Application Immunohistochemistry on Paraffin Sections: Use at 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry on Frozen Sections.
Immunofluorescence of cells in culture.
Flow Cytometry: 1-2 μg per 106 cells.
Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985).
Induction of antibody dependent cellular cyotoxicity (ADCC) (Ortaldo JR et al 1987, J Immunol 138(10):3566-3572).
TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.
Background Gangliosides are membrane-bound, sialic acid-containing glycosphingolipids that play a significant role in determining the nature of tetraspanin interactions. Ganglioside GD3 is produced by the transfer of sialic acid from CMP-sialic acid to GM3. This reaction is catalyzed by the type II membrane protein GD3 Synthase. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. It is found in most normal tissues, and its expression increases under pathological conditions and during development and aging processes. In malignant melanoma cells, Ganglioside GD3 is involved in the upregulation of tyrosine phosphorylation for p130 Cas and paxillin. Ganglioside GD3 also mediates apoptosis, functioning as a regulatory molecule and contributing to mitochondrial damage. The level of Ganglioside GD3 present in a cell plays a significant role in determining cell fate.
Concentration 1.0 mg/ml
General Readings
  1. Cheresh, D.A. et al. (1984) Proc. Natl. Acad. Sci. U.S.A. 81: 5767-5771.
  2. Cheresh, D.A. et al. (1985) Proc. Natl. Acad. Sci. U.S.A. 82: 5155-5159. 
  3.  Ortaldo JR et al. (1987) J Immunol 138:3566-72.

Store undiluted at 2-8°C.
Shelf life: one year from despatch.

Protein A Sepharose Chromatography
Buffer System:
0.02M PB pH 7.6, 0.25M NaCl
0.09% Sodium Azide
Liquid purified IgG fraction
Species Reactivity
Species reactivity (tested):
Reacts specifically with Human GD3 Ganglioside (Cheresh et al., 1984). No cross-reactivity with other Gangliosides purified from neuroblastoma or melanoma cells.

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