AM20243PU-S Dityrosine / DT antibody

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20 µg / €600.00
Please visit the country specific website of Acris Antibodies or contact your local Distributor to buy this product.

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Mouse anti Human Dityrosine / DT 1C3


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  • AM20243PU-S

Product Description for Dityrosine / DT

Mouse anti Human Dityrosine / DT 1C3.
Presentation: Purified
Product is tested for Enzyme Immunoassay, Paraffin Sections, Western blot / Immunoblot.

Properties for Dityrosine / DT

Product Category Primary Antibodies
Quantity 20 µg
Presentation Purified
Reactivity Hu
Applications E, P, WB
Clonality Monoclonal
Clone 1C3
Host Mouse
Isotype IgG2a
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer Acris Antibodies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

3-(p-hydroxyphenyl) Propionic Acid Dimer, KLH-conjugated
Application ELISA (Ref.1,2)
Western blot: 4 µg/ml (Ref.3,5)
Immunohistochemistry on Paraffin Sections (Ref.4,6).
Background Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains.
Dityrosine is one of the specific biomarkers for protein oxidation.
Concentration 0.1 mg/ml (after reconstitution)
General Readings
  1. Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T. Immunochemical detection of protein dityrosine in atherosclerotic lesion of apo-E-deficient mice using a novel monoclonal antibody. Biochem Biophys Res Commun. 2000 Aug 18;275(1):11-5. PubMed PMID: 10944432.
  2. Kato Y, Kitamoto N, Kawai Y, Osawa T. The hydrogen peroxide/copper ion system, but not other metal-catalyzed oxidation systems, produces protein-bound dityrosine. Free Radic Biol Med. 2001 Sep 1;31(5):624-32. PubMed PMID: 11522447.
  3. Atwood CS, Perry G, Zeng H, Kato Y, Jones WD, Ling KQ, et al. Copper mediates dityrosine cross-linking of Alzheimer's amyloid-beta. Biochemistry. 2004 Jan 20;43(2):560-8. PubMed PMID: 14717612.
  4. Shiba Y, Kinoshita T, Chuman H, Taketani Y, Takeda E, Kato Y, et al. Flavonoids as substrates and inhibitors of myeloperoxidase: molecular actions of aglycone and metabolites. Chem Res Toxicol. 2008 Aug;21(8):1600-9. doi: 10.1021/tx8000835. Epub 2008 Jul 12. PubMed PMID: 18620432.
  5. Suderman RJ, Dittmer NT, Kramer KJ, Kanost MR. Model reactions for insect cuticle sclerotization: participation of amino groups in the cross-linking of Manduca sexta cuticle protein MsCP36. Insect Biochem Mol Biol. 2010 Mar;40(3):252-8. doi: 10.1016/j.ibmb.2010.02.008. Epub 2010 Feb 26. PubMed PMID: 20219676.
  6. Kimoto Y, Sugiyama A, Nishinohara M, Asano A, Masuda A, Ochi T, et al. Expressions of protein oxidation markers, dityrosine and advanced oxidation protein products in Cisplatin-induced nephrotoxicity in rats. J Vet Med Sci. 2011 Mar;73(3):403-7. Epub 2010 Nov 1. PubMed PMID: 21048391.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Protein A Chromatography
Buffer System:
10mM PBS, pH 7.4
Lyophilized purified IgG fraction
Restore in 0.2 ml aqua bidest to 0.1 mg/ml of IgG
Specific for free Dityrosine, 3-(p-hydroxyphenyl) Propionic Acid dimer, Dityrosine-BSA conjugate, and Dityrosine in proteins or peptides.
There was no cross reactivity detected for following compounds: Tyrosine, Nitrotyrosine, Chlorotyrosine, Aminotyrosine, Phosphotyrosine, 3-(phydroxyphenyl) Propionic Acid, Phenylalanine, 3,4-Dihydroxyphenylacetic Acid (DOPA).

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